Detailed information of TA system TA049615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 852133..852779
• Replicon: chromosome
• Accession: NZ_CP009757
• Organism: Yersinia pseudotuberculosis strain MD67

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: BZ21_RS04000
• Protein ID: WP_042592977.1
• Coordinates: 852597..852779 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A2A7VE79
• Locus tag: BZ21_RS03995
• Protein ID: WP_042592976.1
• Coordinates: 852133..852552 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ21_RS03945	847267..847839	+	573	WP_042592970.1	DUF1133 family protein	-
BZ21_RS03950	848123..848854	+	732	WP_042592971.1	hypothetical protein	-
BZ21_RS03955	848935..849267	+	333	WP_042592972.1	phage holin, lambda family	-
BZ21_RS03960	849260..849649	+	390	WP_042593087.1	M15 family metallopeptidase	-
BZ21_RS03965	849646..850110	+	465	WP_042592973.1	lysis protein	-
BZ21_RS03970	850136..850351	+	216	WP_042592974.1	hypothetical protein	-
BZ21_RS03975	850430..850612	+	183	WP_042592975.1	hypothetical protein	-
BZ21_RS03980	850849..851217	+	369	WP_038401432.1	hypothetical protein	-
BZ21_RS21650	851248..851853	+	606	WP_071841919.1	terminase small subunit	-
BZ21_RS03995	852133..852552	-	420	WP_042592976.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BZ21_RS04000	852597..852779	-	183	WP_042592977.1	type II toxin-antitoxin system HicA family toxin	Toxin
BZ21_RS04005	852883..854487	+	1605	WP_012303809.1	hypothetical protein	-
BZ21_RS04010	854740..856284	+	1545	WP_042593088.1	DUF1073 domain-containing protein	-
BZ21_RS04015	856241..857122	+	882	WP_042592978.1	head morphogenesis protein SPP1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	826039..876029	49990

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-131)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6796.99 Da
• Isoelectric Point: 11.0775

>T49615 WP_042592977.1 NZ_CP009757:c852779-852597 [Yersinia pseudotuberculosis]
VKSSELIKRLEANGWKLMRVKGSHHQFKHPDFAAVITVPHPKKDVKPGTLRQILKDANLS

Nucleotide

• Length: 183 bp

>T49615 NZ_CP009757:c852779-852597 [Yersinia pseudotuberculosis]
GTGAAAAGTTCGGAGCTGATAAAACGACTCGAAGCAAATGGTTGGAAGTTGATGAGAGTTAAAGGCAGTCATCATCAATT
CAAACATCCTGATTTCGCGGCAGTCATAACAGTACCGCATCCGAAGAAGGACGTTAAACCGGGTACCCTCCGGCAAATTC
TGAAAGATGCAAACCTTAGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15448.21 Da
• Isoelectric Point: 4.7791

>AT49615 WP_042592976.1 NZ_CP009757:c852552-852133 [Yersinia pseudotuberculosis]
MAIYPAYVHVDSDGSASGYFPDVKGCIFAIDAGEDLFAEASSALEAHFEALVSEGIEIPEAHDMPFHYHTNPSNYSDGGQ
WYNVSIDMSKYDGKAERINVTLPHRLIHQIDTIVKVRPEYASRSNFLAEAARKELQKLA

Nucleotide

• Length: 420 bp

>AT49615 NZ_CP009757:c852552-852133 [Yersinia pseudotuberculosis]
ATGGCTATCTATCCCGCATACGTTCATGTCGATAGTGATGGCTCGGCAAGCGGTTATTTCCCTGACGTCAAAGGCTGTAT
TTTTGCCATTGATGCCGGAGAGGATTTATTTGCAGAGGCATCAAGCGCCCTGGAAGCGCACTTTGAGGCGTTGGTGTCTG
AGGGTATTGAGATACCCGAGGCGCATGATATGCCTTTCCACTACCACACCAATCCGAGCAATTACAGTGACGGCGGCCAG
TGGTATAACGTGAGTATTGATATGTCGAAATATGACGGAAAAGCCGAGCGGATTAACGTCACTCTTCCCCACCGGCTGAT
CCATCAAATCGATACCATTGTTAAGGTGCGTCCAGAGTACGCCAGCCGGAGTAATTTTCTAGCCGAAGCAGCACGTAAAG
AATTACAGAAATTAGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A7VE79