Detailed information of TA system TA049613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4767427..4768043
• Replicon: chromosome
• Accession: NZ_CP009756
• Organism: Enterobacter cloacae strain GGT036

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EC036_RS22530
• Protein ID: WP_080292722.1
• Coordinates: 4767427..4767798 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B3JKE9
• Locus tag: EC036_RS22535
• Protein ID: WP_020686881.1
• Coordinates: 4767801..4768043 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC036_RS22515	4764927..4765829	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
EC036_RS22520	4765826..4766461	+	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
EC036_RS22525	4766458..4767387	+	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
EC036_RS22530	4767427..4767798	-	372	WP_080292722.1	type II toxin-antitoxin system VapC family toxin	Toxin
EC036_RS22535	4767801..4768043	-	243	WP_020686881.1	ribbon-helix-helix domain-containing protein	Antitoxin
EC036_RS22540	4768243..4769160	+	918	WP_038420840.1	alpha/beta hydrolase	-
EC036_RS22545	4769173..4770114	-	942	WP_038420841.1	fatty acid biosynthesis protein FabY	-
EC036_RS22550	4770159..4770596	-	438	WP_013099363.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EC036_RS22555	4770593..4771474	-	882	WP_013099364.1	virulence factor BrkB family protein	-
EC036_RS22560	4771468..4772067	-	600	WP_038420842.1	glucose-1-phosphatase	-
EC036_RS22565	4772187..4772987	-	801	WP_020686879.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13625.69 Da
• Isoelectric Point: 6.6407

>T49613 WP_080292722.1 NZ_CP009756:c4767798-4767427 [Enterobacter cloacae]
VNNMAVFDTNILIDLFNNRVEAADAIDHAASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFSGIDEVVTPYQL

Nucleotide

• Length: 372 bp

>T49613 NZ_CP009756:c4767798-4767427 [Enterobacter cloacae]
GTGAACAATATGGCCGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTCGAAGCTGCGGATGCGATTGA
TCATGCCGCGTCACATCGAGCCATTAGCTTAATTACCTGGATGGAGGTGATGGTGGGCGCACGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTCGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTGCGT
GAAAAACATGGAATGAAACTGCCTGATGCCATCATTCTGGCCACAGCCCAGAGCAGAAAATGTCCGTTGATTTCTCGAAA
TACGAAAGATTTTTCGGGTATTGATGAGGTCGTTACGCCTTATCAACTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9369.68 Da
• Isoelectric Point: 4.4076

>AT49613 WP_020686881.1 NZ_CP009756:c4768043-4767801 [Enterobacter cloacae]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRAALGLWGDKKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT49613 NZ_CP009756:c4768043-4767801 [Enterobacter cloacae]
ATGAGTGTTTTGGCGGAACTGGATATGGGCAGAATACTGATCGATTTATCTGATGACGTGATTCAACGGCTGGATAACCT
CAAGCAACTGCGGAATCAGCCGCGCGCTGAGCTGCTACGGGAGGCGATAGAGCTGTATCTCGACCAGCAGAGTTCGTCTG
TTATCCGCGCTGCGCTTGGCTTGTGGGGAGACAAGAAAGAAGACGGGCTGGAGTATGAACGTAAGCTGCGTGAGGAATGG
TGA

Structures

Antitoxin

• AlphaFold DB: A0A8B3JKE9