Detailed information of TA system TA049610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3105464..3106054
• Replicon: chromosome
• Accession: NZ_CP009756
• Organism: Enterobacter cloacae strain GGT036

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EC036_RS14715
• Protein ID: WP_020687576.1
• Coordinates: 3105464..3105796 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A427KRT2
• Locus tag: EC036_RS14720
• Protein ID: WP_020687575.1
• Coordinates: 3105797..3106054 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC036_RS14700	3101623..3102948	+	1326	WP_038420067.1	SidA/IucD/PvdA family monooxygenase	-
EC036_RS14705	3102975..3105164	+	2190	WP_013097826.1	TonB-dependent siderophore receptor	-
EC036_RS14715	3105464..3105796	-	333	WP_020687576.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EC036_RS14720	3105797..3106054	-	258	WP_020687575.1	antitoxin	Antitoxin
EC036_RS14725	3106665..3110564	+	3900	WP_038420069.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11793.68 Da
• Isoelectric Point: 10.5834

>T49610 WP_020687576.1 NZ_CP009756:c3105796-3105464 [Enterobacter cloacae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKVTRLPVVVPVTSGGNFARTAGFTVSLDGAGTKTMGVIRCDQPRTI
DIVARNGKRLERVPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T49610 NZ_CP009756:c3105796-3105464 [Enterobacter cloacae]
ATGGACCGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACAAGGTGACCCGGCTACCGGTAGTGGTTCCCGTCACTAGCGGCGGTAACTTTGCTCGTA
CAGCAGGTTTCACCGTTTCACTGGATGGGGCAGGGACAAAAACAATGGGCGTTATTCGCTGCGACCAGCCCAGAACCATC
GATATAGTGGCCCGGAACGGGAAACGGCTGGAACGCGTACCTGACGCCGTCGTAAATGAGGTGCTTGCCAGGCTGGATGC
GATTTTGAGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9437.90 Da
• Isoelectric Point: 4.3556

>AT49610 WP_020687575.1 NZ_CP009756:c3106054-3105797 [Enterobacter cloacae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSIDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAT
GKEIL

Nucleotide

• Length: 258 bp

>AT49610 NZ_CP009756:c3106054-3105797 [Enterobacter cloacae]
ATGTATACAACTCGCCTGAAAAAGGTTGGCGGTTCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAGACGCTGGAGCT
GTCGATAGACAGCGAAGTGGGCATGACGATTGATAATGGCTGCCTGATAATTGAACCCCAGAAACGGCCACATTATTCGC
TGGAGGAGCTGCTGGCGCAGTGCGATCCGCATGCTGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCAACA
GGAAAGGAGATCCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A427KRT2