Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1131175..1131795 Replicon chromosome
Accession NZ_CP009756
Organism Enterobacter cloacae strain GGT036

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag EC036_RS05325 Protein ID WP_013095889.1
Coordinates 1131175..1131393 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag EC036_RS05330 Protein ID WP_008499288.1
Coordinates 1131421..1131795 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC036_RS05295 1127187..1127447 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
EC036_RS05300 1127450..1127590 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
EC036_RS05305 1127587..1128297 - 711 WP_038418814.1 GNAT family N-acetyltransferase -
EC036_RS05310 1128399..1129859 + 1461 WP_038418815.1 PLP-dependent aminotransferase family protein -
EC036_RS05315 1129831..1130298 - 468 WP_013095887.1 membrane protein -
EC036_RS05320 1130414..1130965 - 552 WP_038418816.1 maltose O-acetyltransferase -
EC036_RS05325 1131175..1131393 - 219 WP_013095889.1 hemolysin expression modulator Hha Toxin
EC036_RS05330 1131421..1131795 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
EC036_RS05335 1132308..1135454 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit -
EC036_RS05340 1135477..1136670 - 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T49600 WP_013095889.1 NZ_CP009756:c1131393-1131175 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T49600 NZ_CP009756:c1131393-1131175 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGCCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCGCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT49600 WP_008499288.1 NZ_CP009756:c1131795-1131421 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT49600 NZ_CP009756:c1131795-1131421 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACATCGGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References