Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 629959..630535 Replicon chromosome
Accession NZ_CP009756
Organism Enterobacter cloacae strain GGT036

Toxin (Protein)


Gene name shpA Uniprot ID A0A5E1APA0
Locus tag EC036_RS02980 Protein ID WP_023620650.1
Coordinates 629959..630246 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A5E1AP18
Locus tag EC036_RS02985 Protein ID WP_023620649.1
Coordinates 630233..630535 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC036_RS02955 625023..625718 + 696 WP_038418621.1 winged helix-turn-helix domain-containing protein -
EC036_RS02960 625921..626391 + 471 WP_013095432.1 MarR family transcriptional regulator -
EC036_RS02965 626388..627455 + 1068 WP_086528209.1 HlyD family secretion protein -
EC036_RS02970 627445..628521 + 1077 WP_038418624.1 DUF2955 domain-containing protein -
EC036_RS02975 628491..629789 - 1299 WP_038418625.1 DUF445 domain-containing protein -
EC036_RS02980 629959..630246 + 288 WP_023620650.1 BrnT family toxin Toxin
EC036_RS02985 630233..630535 + 303 WP_023620649.1 BrnA antitoxin family protein Antitoxin
EC036_RS02990 630566..631204 - 639 WP_038418626.1 LysE family translocator -
EC036_RS02995 631253..631996 - 744 WP_038418627.1 AraC family transcriptional regulator -
EC036_RS03000 632133..633503 + 1371 WP_038418628.1 NAD-dependent succinate-semialdehyde dehydrogenase -
EC036_RS03005 633545..633865 - 321 WP_013095439.1 hypothetical protein -
EC036_RS03010 633865..634410 - 546 WP_013095440.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11246.60 Da        Isoelectric Point: 6.8813

>T49598 WP_023620650.1 NZ_CP009756:629959-630246 [Enterobacter cloacae]
MPTEYEWDSDKAKSNQQKHGIRFEDAVLVFDDPYHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGGEIIRIIS
ARKADRKERNRYEHR

Download         Length: 288 bp

>T49598 NZ_CP009756:629959-630246 [Enterobacter cloacae]
ATGCCAACGGAGTATGAGTGGGATAGCGATAAGGCGAAAAGTAACCAGCAAAAACATGGCATACGCTTTGAGGATGCGGT
TCTGGTTTTCGACGATCCTTATCATTTGTCTGTTCAGGATCGCTATGAGAACGGTGAGTTTCGCTGGCAAACCATCGGCC
TTGTGCAAGGCGTCATTGTTATCCTGGTCGCACATACGGTACGTTTCGAAAGCGGAGGCGAAATTATTCGTATCATTAGC
GCACGTAAGGCGGACCGTAAAGAGAGGAACCGTTATGAGCATCGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11414.05 Da        Isoelectric Point: 10.3694

>AT49598 WP_023620649.1 NZ_CP009756:630233-630535 [Enterobacter cloacae]
MSIVKHKRGSSPEVSARRDAELKALAEKPEAEIDYSDIPATDDKQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT49598 NZ_CP009756:630233-630535 [Enterobacter cloacae]
ATGAGCATCGTTAAACATAAACGAGGTAGTTCGCCTGAGGTTAGCGCTCGTCGTGACGCTGAGCTAAAAGCATTAGCTGA
AAAACCAGAAGCAGAAATCGATTACAGCGATATTCCTGCCACGGACGATAAACAGTGGTCCGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTTAAAACGCAGGCATCTGTACGCATTGACGCCGACGTGATGGAGTGGCTGAAACGGCCTGGAAAGGGT
TACCAGACGCGGCTAAACGCCATATTGCGTGAAGCGATGCTGCGCGATCAAAACAAAAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1APA0

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP18

References