Detailed information of TA system TA049597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 623328..624122
• Replicon: chromosome
• Accession: NZ_CP009756
• Organism: Enterobacter cloacae strain GGT036

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: EC036_RS02935
• Protein ID: WP_038418617.1
• Coordinates: 623328..623849 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A5E1AP76
• Locus tag: EC036_RS02940
• Protein ID: WP_013095428.1
• Coordinates: 623853..624122 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC036_RS02910	618655..618816	+	162	WP_013095422.1	DUF1127 domain-containing protein	-
EC036_RS02915	618903..620291	-	1389	WP_038418613.1	PLP-dependent aminotransferase family protein	-
EC036_RS02920	620394..620873	+	480	WP_038418614.1	carboxymuconolactone decarboxylase family protein	-
EC036_RS02925	620860..621762	-	903	WP_023620661.1	LysR family transcriptional regulator	-
EC036_RS02930	621863..623278	+	1416	WP_038418616.1	aldehyde dehydrogenase family protein	-
EC036_RS02935	623328..623849	-	522	WP_038418617.1	GNAT family N-acetyltransferase	Toxin
EC036_RS02940	623853..624122	-	270	WP_013095428.1	DUF1778 domain-containing protein	Antitoxin
EC036_RS02945	624337..624660	+	324	WP_038418618.1	antibiotic biosynthesis monooxygenase	-
EC036_RS02950	624653..625051	+	399	WP_038418619.1	hypothetical protein	-
EC036_RS02955	625023..625718	+	696	WP_038418621.1	winged helix-turn-helix domain-containing protein	-
EC036_RS02960	625921..626391	+	471	WP_013095432.1	MarR family transcriptional regulator	-
EC036_RS02965	626388..627455	+	1068	WP_086528209.1	HlyD family secretion protein	-
EC036_RS02970	627445..628521	+	1077	WP_038418624.1	DUF2955 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-157)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19352.30 Da
• Isoelectric Point: 9.4920

>T49597 WP_038418617.1 NZ_CP009756:c623849-623328 [Enterobacter cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRFQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNGSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Nucleotide

• Length: 522 bp

>T49597 NZ_CP009756:c623849-623328 [Enterobacter cloacae]
GTGAATAACATGAAAATAGGGATTTTTTCAGATGACGTTGAGTATGATCTAAGCCATTTCGATTGCGGAGAAGAGAGCCT
AAACACCTTTCTGACGGCGCACCTGAAACGCCAGCATCGTGGGAAATTTTTGCGGGGTTACGTTCTTGTCGCATCCGGGG
AAAAGCCGCGGGTGTTGGGTTATTACACGCTGTCCGGGAGCTGCTTTGAAAAGGCGTACCTTCCTTCTAAAACCCAGCAG
AAACGAGTGCCTTACAAAAATGTTCCCAGCGTCACGCTAGGCCGCCTGGCGATAGATAAACGCTTCCAGGGGCAAGGATT
GGGGGAGTTGCTGGTCACCCATGCTCTGAAAACGGTCTACCTGGCGTCTTTTGCCGTGGGTATCCACGGGATATTCGTAG
AAGCGCTGAACGGCAGCGCCAAAAACTTTTACCTTAAACTGGGGTTTATCGCCCTTCAGGCAGAGAATGAAAATACCCTC
TTCCTGCCGACTAAAACCATTGAAAGACTGTTTGCAGATTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10177.54 Da
• Isoelectric Point: 5.8652

>AT49597 WP_013095428.1 NZ_CP009756:c624122-623853 [Enterobacter cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Nucleotide

• Length: 270 bp

>AT49597 NZ_CP009756:c624122-623853 [Enterobacter cloacae]
ATGTCAGCACTAAAGAAACAGCGTATCGATCTACGCTTAACCGAAGACGACAAAAGCCTGATCGAAGAAGCAGCGGCAAT
GACGAACCAGTCAATTTCACAATTCATGGTAAGTACGGCCTCCGAACGCGCAGCAGAAGTGATTGAACAGCACCGCCGCC
TGATCCTCAACGAGGCATCCTGGAACCAGGTGATGGATGCGATCAGTAACCCGCCAACACCAAATGAACGACTGAAGCGA
GCAGCCAGGCGTCTCCAGGATCTGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AP76