Detailed information of TA system TA049596

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 588438..589221
• Replicon: chromosome
• Accession: NZ_CP009756
• Organism: Enterobacter cloacae strain GGT036

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: EC036_RS02770
• Protein ID: WP_038418596.1
• Coordinates: 588847..589221 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: EC036_RS02765
• Protein ID: WP_038418595.1
• Coordinates: 588438..588797 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC036_RS02720	584062..584739	+	678	WP_038418587.1	WYL domain-containing protein	-
EC036_RS02725	584847..585257	+	411	WP_052123661.1	hypothetical protein	-
EC036_RS02730	585311..585670	+	360	WP_038418588.1	hypothetical protein	-
EC036_RS02735	585756..585968	+	213	WP_038418589.1	hypothetical protein	-
EC036_RS02740	586078..586899	+	822	WP_038418590.1	DUF945 domain-containing protein	-
EC036_RS02745	586978..587457	+	480	WP_038418591.1	antirestriction protein	-
EC036_RS02750	587468..587701	+	234	WP_038418592.1	hypothetical protein	-
EC036_RS02755	587698..588180	+	483	WP_038418593.1	DNA repair protein RadC	-
EC036_RS02760	588194..588415	+	222	WP_038418594.1	DUF987 family protein	-
EC036_RS02765	588438..588797	+	360	WP_038418595.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EC036_RS02770	588847..589221	+	375	WP_038418596.1	TA system toxin CbtA family protein	Toxin
EC036_RS02775	589342..589605	+	264	Protein_537	transcriptional regulator	-
EC036_RS02780	589958..590476	+	519	WP_048968430.1	hypothetical protein	-
EC036_RS02785	590625..591539	-	915	WP_038418598.1	hypothetical protein	-
EC036_RS02790	592123..592992	-	870	Protein_540	HNH endonuclease	-
EC036_RS23690	593293..593748	+	456	WP_080292697.1	SymE family type I addiction module toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	fosA	-	555685..600699	45014

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (9-112)

Antitoxin

• Antitoxin CbeA (16-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14175.23 Da
• Isoelectric Point: 9.8616

>T49596 WP_038418596.1 NZ_CP009756:588847-589221 [Enterobacter cloacae]
MQIQYLSPQRAASSRPSSVEIWRKLLEYLLEQHYGLTLNDTPFGNDSVIQKYIDAGISLCDAVNFIVEKYDLVRTDRQGF
SVKEQFPFIGSIDMLRARKATGLMTRKGYKTVTDITSSRFSGRK

Nucleotide

• Length: 375 bp

>T49596 NZ_CP009756:588847-589221 [Enterobacter cloacae]
ATGCAAATACAATATCTGTCCCCTCAAAGGGCGGCATCGTCACGTCCGTCATCAGTCGAAATCTGGCGAAAATTACTGGA
GTATCTGCTGGAGCAACACTATGGCCTTACCCTCAACGACACACCGTTTGGCAACGACAGTGTGATCCAGAAGTACATTG
ATGCGGGTATCTCACTGTGTGATGCGGTGAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGATCGTCAAGGCTTC
AGCGTAAAAGAGCAGTTTCCGTTTATCGGCAGCATTGATATGCTCCGCGCCCGGAAGGCTACCGGGCTGATGACCCGCAA
GGGATATAAAACCGTTACTGACATCACCAGTAGCAGATTCAGCGGCAGGAAATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13368.48 Da
• Isoelectric Point: 7.7729

>AT49596 WP_038418595.1 NZ_CP009756:588438-588797 [Enterobacter cloacae]
MQKATRLINHNIADTWWGLRRNVTPCFGARLVQEGNHLYYLADRASIAGTFNDADLRHLDQAFPVLMKRMELMLTSSELT
PHIQRCLTLHAKGLICEADTLGSCGYLHIVIYPTSAITA

Nucleotide

• Length: 360 bp

>AT49596 NZ_CP009756:588438-588797 [Enterobacter cloacae]
ATGCAGAAAGCAACCCGTTTAATCAACCATAACATCGCGGACACCTGGTGGGGCCTCAGACGCAACGTCACGCCGTGTTT
TGGTGCACGGCTGGTACAGGAGGGTAACCACCTGTATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTCAATGACG
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAGCGGATGGAGCTCATGCTGACCAGCAGCGAGCTCACA
CCTCACATTCAGCGCTGCCTCACGCTCCACGCAAAAGGGCTGATATGTGAGGCCGATACGCTCGGCTCCTGTGGCTATTT
GCATATCGTAATTTATCCGACATCTGCAATAACCGCATAA