Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1262917..1263833 Replicon chromosome
Accession NZ_CP009748
Organism Bacillus subtilis strain ATCC 13952

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A7U5AVM4
Locus tag KS08_RS06475 Protein ID WP_013351967.1
Coordinates 1263087..1263833 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag KS08_RS06470 Protein ID WP_003154807.1
Coordinates 1262917..1263087 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KS08_RS06435 1258058..1259821 + 1764 WP_014470496.1 hypothetical protein -
KS08_RS06440 1259833..1260159 + 327 WP_014470495.1 XkdW family protein -
KS08_RS06445 1260159..1260323 + 165 WP_014470494.1 XkdX family protein -
KS08_RS06450 1260378..1261175 + 798 WP_014470493.1 hypothetical protein -
KS08_RS06455 1261229..1261492 + 264 WP_013351964.1 hemolysin XhlA family protein -
KS08_RS06460 1261506..1261769 + 264 WP_014470492.1 phage holin -
KS08_RS06465 1261783..1262661 + 879 WP_014470491.1 N-acetylmuramoyl-L-alanine amidase -
KS08_RS06470 1262917..1263087 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
KS08_RS06475 1263087..1263833 - 747 WP_013351967.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
KS08_RS06480 1263941..1264939 - 999 WP_013351968.1 inorganic phosphate transporter -
KS08_RS06485 1264952..1265569 - 618 WP_003154804.1 DUF47 domain-containing protein -
KS08_RS06490 1265854..1267170 - 1317 WP_013351969.1 amino acid permease -
KS08_RS06495 1267494..1268444 + 951 WP_013351970.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29054.53 Da        Isoelectric Point: 4.6839

>T49583 WP_013351967.1 NZ_CP009748:c1263833-1263087 [Bacillus subtilis]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T49583 NZ_CP009748:c1263833-1263087 [Bacillus subtilis]
ATGCTTTTGTTTTATCAAATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTATGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGGCAATACTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATATTGGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCAGAAGGCATCAGCAGACTGCTGGCCGCTTATGCAGAAAAAATGAATGTCACGGCTTCATTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACAGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGCCTTAA
CCGGAAAGACGTCTACTATGATCAAAAAGGAAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCAGTATGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT49583 WP_003154807.1 NZ_CP009748:c1263087-1262917 [Bacillus subtilis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT49583 NZ_CP009748:c1263087-1262917 [Bacillus subtilis]
ATGGAGCAGGCGTTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGCTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCACACCGCCAGAATTTTCAAAGAAAATGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U5AVM4

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References