Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 462478..463115 Replicon chromosome
Accession NZ_CP009748
Organism Bacillus subtilis strain ATCC 13952

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag KS08_RS02295 Protein ID WP_003156187.1
Coordinates 462765..463115 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag KS08_RS02290 Protein ID WP_003156188.1
Coordinates 462478..462759 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KS08_RS02270 458844..459443 - 600 WP_013351092.1 rhomboid family intramembrane serine protease -
KS08_RS02275 459536..459901 + 366 WP_013351093.1 holo-ACP synthase -
KS08_RS02280 460066..461073 + 1008 WP_013351094.1 outer membrane lipoprotein carrier protein LolA -
KS08_RS02285 461190..462359 + 1170 WP_013351095.1 alanine racemase -
KS08_RS02290 462478..462759 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
KS08_RS02295 462765..463115 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
KS08_RS02300 463236..464057 + 822 WP_013351096.1 STAS domain-containing protein -
KS08_RS02305 464062..464427 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
KS08_RS02310 464430..464831 + 402 WP_013351097.1 anti-sigma regulatory factor -
KS08_RS02315 464843..465850 + 1008 WP_013351098.1 PP2C family protein-serine/threonine phosphatase -
KS08_RS02320 465914..466243 + 330 WP_014469786.1 anti-sigma factor antagonist -
KS08_RS02325 466240..466722 + 483 WP_003156173.1 anti-sigma B factor RsbW -
KS08_RS02330 466688..467476 + 789 WP_013351101.1 RNA polymerase sigma factor SigB -
KS08_RS02335 467476..468078 + 603 WP_014469787.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T49582 WP_003156187.1 NZ_CP009748:462765-463115 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T49582 NZ_CP009748:462765-463115 [Bacillus subtilis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT49582 WP_003156188.1 NZ_CP009748:462478-462759 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT49582 NZ_CP009748:462478-462759 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAGGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATTTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACGGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References