Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5256144..5256786 Replicon chromosome
Accession NZ_CP009746
Organism Bacillus mycoides strain WSBC10204

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BWEI_RS25900 Protein ID WP_000635965.1
Coordinates 5256144..5256494 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BWEI_RS25905 Protein ID WP_000004570.1
Coordinates 5256499..5256786 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWEI_RS25890 5253067..5253522 - 456 WP_016095131.1 SprT family protein -
BWEI_RS29785 5253733..5253849 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BWEI_RS25895 5253907..5256075 - 2169 WP_002029442.1 RNA-binding transcriptional accessory protein -
BWEI_RS25900 5256144..5256494 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BWEI_RS25905 5256499..5256786 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BWEI_RS25910 5257098..5258267 - 1170 WP_002029440.1 alanine racemase -
BWEI_RS25915 5258385..5259335 - 951 WP_002124820.1 outer membrane lipoprotein carrier protein LolA -
BWEI_RS25920 5259492..5259851 - 360 WP_002029438.1 holo-ACP synthase -
BWEI_RS29270 5259943..5260515 + 573 WP_080683328.1 rhomboid family intramembrane serine protease -
BWEI_RS25930 5260508..5261470 - 963 WP_002029436.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5250946..5251659 713


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T49581 WP_000635965.1 NZ_CP009746:c5256494-5256144 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T49581 NZ_CP009746:c5256494-5256144 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT49581 WP_000004570.1 NZ_CP009746:c5256786-5256499 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT49581 NZ_CP009746:c5256786-5256499 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References