Detailed information of TA system TA049580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2383388..2384366
• Replicon: chromosome
• Accession: NZ_CP009746
• Organism: Bacillus mycoides strain WSBC10204

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: R8N1U0
• Locus tag: BWEI_RS12045
• Protein ID: WP_002032139.1
• Coordinates: 2383626..2384366 (-)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: BWEI_RS12040
• Protein ID: WP_000588712.1
• Coordinates: 2383388..2383513 (-)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BWEI_RS12020	2379156..2380350	+	1195	Protein_2453	collagen-like protein	-
BWEI_RS12025	2381007..2382476	-	1470	WP_002032146.1	beta-Ala-His dipeptidase	-
BWEI_RS12030	2382748..2383116	+	369	WP_033709111.1	YxeA family protein	-
BWEI_RS30210	2383136..2383312	-	177	WP_002032141.1	hypothetical protein	-
BWEI_RS12040	2383388..2383513	-	126	WP_000588712.1	hypothetical protein	Antitoxin
BWEI_RS12045	2383626..2384366	-	741	WP_002032139.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BWEI_RS12050	2384706..2385443	-	738	WP_002032137.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BWEI_RS12055	2385597..2386073	-	477	WP_002017049.1	MarR family winged helix-turn-helix transcriptional regulator	-
BWEI_RS12060	2386347..2386979	+	633	WP_002032134.1	glutamine amidotransferase	-
BWEI_RS12065	2387156..2387569	-	414	WP_002032132.1	VOC family protein	-
BWEI_RS12070	2387688..2388398	-	711	Protein_2463	class I SAM-dependent methyltransferase	-
BWEI_RS12075	2388504..2389265	-	762	WP_033709109.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28482.11 Da
• Isoelectric Point: 8.3034

>T49580 WP_002032139.1 NZ_CP009746:c2384366-2383626 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFTIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T49580 NZ_CP009746:c2384366-2383626 [Bacillus mycoides]
TTGACAATCTCTAACATTCGAATTGGCTTATTTATTTTAGCAATCGTCTTTATTGTTCTTGTTTTCTTTTATTGGAGAAA
TGAAGAGTTGTACGAGGAGAAGAAACAACGAATTCGAAAAACGTGGTACGGTTTGTTCATAACATCAGTCACAGTTTATT
TCACGATAAAAGGAATAGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCGATGGTAATTTTCGTTGATATTGCA
TTCATTTTGACACCAAATATTTCAGAAATATGGGGCGCAAAATTCAGCGATATTGGAAAGACGGTCCAATCAATTAAAAG
GTCATTAATTGCTTCAAAAGCAAGAGGAGAAATATATACGACAATTATTCAAAATGTGAATCCAACAGCCTTTGGGACAA
TGGAATGGCATACGGAAGAGGAATATACAAAAAGCTTAAACACATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAA
ATTGTTGTATTTGAAGCGGTGAAGGAATTAAATACAAACTTTCGTGGTATCCGCTCTCAATTTAGTATTATTGTTCCATT
AGAACACATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTTAGTG
ATGTTTTCATTGTTATTGATGGGAAGAAAAATAACCTTCAAGATCGAGATTTTGAAAATGTATATAATTTAACAATTCAT
CATAGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT49580 WP_000588712.1 NZ_CP009746:c2383513-2383388 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT49580 NZ_CP009746:c2383513-2383388 [Bacillus mycoides]
GTGATAAAAGATTATGACAATGATTTTTTTAAAGAAGATAATGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
TTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Toxin

• AlphaFold DB: R8N1U0

Antitoxin

• AlphaFold DB: A0A366GQT1