Detailed information of TA system TA049573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 538408..539063
• Replicon: chromosome
• Accession: NZ_CP009743
• Organism: Burkholderia contaminans strain MS14

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: NL30_RS02265
• Protein ID: WP_047849666.1
• Coordinates: 538644..539063 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R4C038
• Locus tag: NL30_RS02260
• Protein ID: WP_046548594.1
• Coordinates: 538408..538647 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NL30_RS02235	533661..534056	-	396	WP_047851020.1	type II toxin-antitoxin system VapC family toxin	-
NL30_RS02240	534053..534292	-	240	WP_010089452.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
NL30_RS02245	534390..535073	-	684	WP_047849664.1	Fe2+-dependent dioxygenase	-
NL30_RS02250	535070..535849	-	780	WP_046548296.1	sel1 repeat family protein	-
NL30_RS02255	535853..538096	-	2244	WP_047849665.1	TonB-dependent siderophore receptor	-
NL30_RS02260	538408..538647	+	240	WP_046548594.1	Arc family DNA-binding protein	Antitoxin
NL30_RS02265	538644..539063	+	420	WP_047849666.1	type II toxin-antitoxin system VapC family toxin	Toxin
NL30_RS02270	539180..540475	-	1296	WP_039360985.1	aspartate carbamoyltransferase	-
NL30_RS02275	540709..540843	+	135	WP_006485835.1	entericidin A/B family lipoprotein	-
NL30_RS02280	540943..541491	-	549	WP_046548293.1	hypothetical protein	-
NL30_RS02285	541700..542677	-	978	WP_122476337.1	DMT family transporter	-
NL30_RS02290	542864..543658	-	795	WP_047849668.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15169.45 Da
• Isoelectric Point: 5.7471

>T49573 WP_047849666.1 NZ_CP009743:538644-539063 [Burkholderia contaminans]
MILVDTNVMSEPLRREPSAAVIEWLDAQNVETLFLAAISLGEMRFGVAALPEGRRRDWLHHSIEQRVVPMFRGRILPFDD
AASKAYASIRARARASGSTIAPADVFIAATAEANGLIIATRDVAPFEAVGLRVIDPWAS

Nucleotide

• Length: 420 bp

>T49573 NZ_CP009743:538644-539063 [Burkholderia contaminans]
ATGATCCTTGTTGATACGAACGTGATGTCCGAGCCGCTGCGGCGTGAGCCGAGCGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAACGTCGAAACGTTGTTTCTTGCTGCGATCAGTCTTGGCGAAATGCGCTTCGGCGTGGCGGCGCTCCCGGAAGGGC
GCAGGCGCGACTGGCTTCACCACAGCATCGAGCAACGCGTCGTGCCGATGTTTCGCGGCAGGATTCTGCCGTTCGACGAT
GCGGCGAGCAAAGCGTATGCGAGCATTCGAGCGCGTGCGCGTGCCTCGGGCAGTACGATCGCGCCTGCAGATGTCTTTAT
TGCCGCGACGGCCGAGGCGAACGGATTGATCATTGCGACGCGCGACGTTGCGCCATTCGAGGCTGTCGGCCTGCGGGTGA
TTGATCCATGGGCGTCGTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8776.06 Da
• Isoelectric Point: 6.4684

>AT49573 WP_046548594.1 NZ_CP009743:538408-538647 [Burkholderia contaminans]
MPVITVRNLPDEVHRALRIRAAQHGRSTEAEVRDILEQAVLPGGRLKLGTLLAEIGREAGGVEFDVRRDKTLTDPMSFE

Nucleotide

• Length: 240 bp

>AT49573 NZ_CP009743:538408-538647 [Burkholderia contaminans]
ATGCCCGTGATTACTGTTCGAAATTTGCCCGATGAAGTGCACCGCGCGCTCCGAATTCGTGCTGCGCAGCATGGGCGTAG
CACTGAAGCCGAGGTGCGTGACATCCTGGAGCAGGCCGTTCTGCCGGGCGGCCGGCTGAAGCTGGGAACGCTGCTGGCTG
AAATCGGACGGGAGGCGGGCGGCGTCGAATTCGACGTTCGGCGCGACAAGACGCTAACCGACCCGATGAGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A4R4C038