Detailed information of TA system TA049528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 2667594..2668240
• Replicon: chromosome
• Accession: NZ_CP009715
• Organism: Yersinia pestis Pestoides F

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3G5L3F2
• Locus tag: BZ18_RS13470
• Protein ID: WP_002214976.1
• Coordinates: 2668058..2668240 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0U1R391
• Locus tag: BZ18_RS13465
• Protein ID: WP_002211129.1
• Coordinates: 2667594..2668013 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ18_RS13445	2662665..2663660	+	996	WP_002211125.1	substrate-binding domain-containing protein	-
BZ18_RS13450	2663726..2665303	+	1578	WP_002211126.1	sugar ABC transporter ATP-binding protein	-
BZ18_RS13455	2665306..2666328	+	1023	WP_002214974.1	ABC transporter permease	-
BZ18_RS13460	2666377..2667336	+	960	WP_002211128.1	sugar kinase	-
BZ18_RS13465	2667594..2668013	-	420	WP_002211129.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
BZ18_RS13470	2668058..2668240	-	183	WP_002214976.1	type II toxin-antitoxin system HicA family toxin	Toxin
BZ18_RS13475	2668447..2669229	-	783	WP_002211130.1	flagellar type III secretion system protein FliR	-
BZ18_RS13480	2669232..2669501	-	270	WP_002211131.1	flagellar biosynthesis protein FliQ	-
BZ18_RS13485	2669607..2670347	-	741	WP_002220494.1	flagellar type III secretion system pore protein FliP	-
BZ18_RS13490	2670428..2670964	-	537	WP_002211133.1	flagellar biosynthetic protein FliO	-
BZ18_RS13495	2670964..2671380	-	417	WP_011906247.1	flagellar motor switch protein FliN	-
BZ18_RS13500	2671377..2672378	-	1002	WP_002211135.1	flagellar motor switch protein FliM	-
BZ18_RS13505	2672384..2672854	-	471	WP_002227961.1	flagellar basal body-associated protein FliL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-131)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6783.99 Da
• Isoelectric Point: 10.8877

>T49528 WP_002214976.1 NZ_CP009715:c2668240-2668058 [Yersinia pestis Pestoides F]
VKSSELIKLLETNGWKLMRVKGSHHQFKHPDFAAVITVPHPKKDVKPGTLRQILKDANLS

Nucleotide

• Length: 183 bp

>T49528 NZ_CP009715:c2668240-2668058 [Yersinia pestis Pestoides F]
GTGAAAAGTTCGGAGCTGATAAAACTGCTCGAAACAAATGGTTGGAAGTTGATGAGAGTTAAAGGCAGTCATCATCAATT
CAAACATCCTGATTTCGCGGCAGTCATAACAGTACCGCATCCCAAAAAGGATGTTAAACCGGGAACCCTCCGGCAAATTC
TGAAAGATGCAAACCTTAGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15549.42 Da
• Isoelectric Point: 4.7731

>AT49528 WP_002211129.1 NZ_CP009715:c2668013-2667594 [Yersinia pestis Pestoides F]
MAIYPAYVHVDNDGSASGYFPDVKGCIFAINAGEDLFAEASSALDAHFEALVSEGIEIPEAHDMPYHVYRNPCDYADGGQ
WYNVNIDMSKYDGKVERINVTLPHRLIHQIDTIVKVRPEYASRSNFLAEAARKELQKLA

Nucleotide

• Length: 420 bp

>AT49528 NZ_CP009715:c2668013-2667594 [Yersinia pestis Pestoides F]
ATGGCTATCTATCCCGCCTATGTTCACGTTGATAATGATGGCTCGGCAAGCGGTTATTTCCCCGATGTCAAAGGCTGTAT
TTTTGCCATCAATGCCGGGGAGGATTTATTTGCAGAAGCATCAAGCGCCCTGGATGCACACTTTGAAGCTTTGGTGTCTG
AAGGTATCGAAATTCCTGAAGCTCATGACATGCCTTACCACGTTTACCGTAACCCATGCGATTACGCTGATGGCGGACAG
TGGTATAACGTGAATATTGATATGTCGAAATATGATGGAAAAGTTGAACGAATTAACGTCACTCTTCCGCACCGGCTGAT
TCATCAAATCGATACTATTGTTAAAGTGCGTCCCGAGTATGCCAGCCGAAGCAATTTTCTAGCCGAAGCAGCACGTAAAG
AATTACAGAAATTGGCGTAA

Structures

Toxin

• AlphaFold DB: A0A3G5L3F2

Antitoxin

• AlphaFold DB: A0A0U1R391