Detailed information of TA system TA049516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 61801..62335
• Replicon: plasmid pCD
• Accession: NZ_CP009713
• Organism: Yersinia pestis Pestoides F

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: BZ18_RS00400
• Protein ID: WP_002213360.1
• Coordinates: 62036..62335 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: BZ18_RS00395
• Protein ID: WP_002224342.1
• Coordinates: 61801..62043 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ18_RS00385	58500..59666	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
BZ18_RS00390	59663..60628	+	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
BZ18_RS24555	61083..61256	-	174	WP_002213357.1	hypothetical protein	-
BZ18_RS23515	61343..61635	+	293	Protein_79	IS6 family transposase	-
BZ18_RS00395	61801..62043	+	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BZ18_RS00400	62036..62335	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BZ18_RS00405	62475..63134	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
BZ18_RS00410	63328..63720	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
BZ18_RS00415	63783..64427	-	645	Protein_84	IS3 family transposase	-
BZ18_RS00425	64530..65702	+	1173	WP_002220893.1	IS21 family transposase	-
BZ18_RS00430	65702..66100	+	399	Protein_86	ATP-binding protein	-
BZ18_RS00435	66477..66902	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yadA / yadA / yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD / yopM / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH	1..71507	71507

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T49516 WP_002213360.1 NZ_CP009713:62036-62335 [Yersinia pestis Pestoides F]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T49516 NZ_CP009713:62036-62335 [Yersinia pestis Pestoides F]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT49516 WP_002224342.1 NZ_CP009713:61801-62043 [Yersinia pestis Pestoides F]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT49516 NZ_CP009713:61801-62043 [Yersinia pestis Pestoides F]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6