Detailed information of TA system TA049502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 15332..15866
• Replicon: plasmid pYAC_1
• Accession: NZ_CP009711
• Organism: Yersinia pseudotuberculosis IP 32953 strain IP32953

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: BZ17_RS00250
• Protein ID: WP_002213360.1
• Coordinates: 15567..15866 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: BZ17_RS00245
• Protein ID: WP_012414146.1
• Coordinates: 15332..15574 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ17_RS00230	11897..13063	+	1167	WP_002220902.1	plasmid-partitioning protein SopA	-
BZ17_RS00235	13060..14025	+	966	WP_011191369.1	ParB/RepB/Spo0J family plasmid partition protein	-
BZ17_RS24170	14185..14466	+	282	WP_157868821.1	hypothetical protein	-
BZ17_RS24285	14616..14789	-	174	WP_002213357.1	hypothetical protein	-
BZ17_RS23355	14874..15166	+	293	Protein_20	IS6 family transposase	-
BZ17_RS00245	15332..15574	+	243	WP_012414146.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
BZ17_RS00250	15567..15866	+	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BZ17_RS00255	16006..16665	-	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
BZ17_RS00260	16859..17251	+	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
BZ17_RS00265	17314..17958	-	645	Protein_25	IS3 family transposase	-
BZ17_RS00275	18061..19233	+	1173	WP_011191364.1	IS21 family transposase	-
BZ17_RS00280	19233..19631	+	399	Protein_27	ATP-binding protein	-
BZ17_RS00285	20009..20434	+	426	WP_002213267.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopD / sycT / yopT / yopK/yopQ / yopE / sycE/yerA / sycH / yadA / yopO/ypkA / yopJ/yopP / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscP / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB	1..68525	68525

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T49502 WP_002213360.1 NZ_CP009711:15567-15866 [Yersinia pseudotuberculosis IP 32953]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T49502 NZ_CP009711:15567-15866 [Yersinia pseudotuberculosis IP 32953]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8807.12 Da
• Isoelectric Point: 6.8783

>AT49502 WP_012414146.1 NZ_CP009711:15332-15574 [Yersinia pseudotuberculosis IP 32953]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKGTSV

Nucleotide

• Length: 243 bp

>AT49502 NZ_CP009711:15332-15574 [Yersinia pseudotuberculosis IP 32953]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGGTACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8