49500

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 1927229..1927862 Replicon chromosome
Accession NZ_CP009709
Organism Bacillus coagulans DSM

Toxin (Protein)


Gene name mazF Uniprot ID G2TIM0
Locus tag BF29_RS09755 Protein ID WP_013860680.1
Coordinates 1927229..1927579 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G2TIL9
Locus tag BF29_RS09760 Protein ID WP_014096808.1
Coordinates 1927584..1927862 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BF29_RS09715 1922414..1923193 - 780 WP_029142785.1 RNA polymerase sigma factor SigB -
BF29_RS09720 1923171..1923641 - 471 WP_017553287.1 anti-sigma B factor RsbW -
BF29_RS09725 1923645..1923974 - 330 WP_013860674.1 anti-sigma factor antagonist -
BF29_RS09730 1924043..1925053 - 1011 WP_029142786.1 PP2C family protein-serine/threonine phosphatase -
BF29_RS09735 1925068..1925469 - 402 WP_029142787.1 anti-sigma regulatory factor -
BF29_RS09740 1925474..1925830 - 357 WP_017553290.1 STAS domain-containing protein -
BF29_RS09745 1925834..1926661 - 828 WP_029142788.1 RsbT co-antagonist protein RsbRA -
BF29_RS09755 1927229..1927579 - 351 WP_013860680.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BF29_RS09760 1927584..1927862 - 279 WP_014096808.1 hypothetical protein Antitoxin
BF29_RS09765 1928015..1929166 - 1152 WP_029142789.1 alanine racemase -
BF29_RS09770 1929368..1930384 - 1017 WP_013860683.1 outer membrane lipoprotein carrier protein LolA -
BF29_RS09775 1930525..1930875 - 351 WP_029142790.1 holo-ACP synthase -
BF29_RS09780 1931157..1931756 + 600 WP_029142791.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12968.01 Da        Isoelectric Point: 4.8998

>T49500 WP_013860680.1 NZ_CP009709:c1927579-1927229 [Bacillus coagulans DSM 1 = ATCC 7050]
MIVKRGDVYFADLSPVVGSEQGGVRPVLILQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGLERDSVILLEQI
RTIDKQRLTDKITHLDEEVMEKIDDALQISLGLVEF

Download         Length: 351 bp

>T49500 NZ_CP009709:c1927579-1927229 [Bacillus coagulans DSM 1 = ATCC 7050]
TTGATTGTTAAGCGTGGAGACGTGTACTTCGCCGATCTATCTCCGGTGGTCGGCTCCGAGCAGGGAGGCGTACGTCCTGT
CCTCATTTTACAAAACGACATTGGAAACCGCTTTAGCCCCACTGTCATTGTTGCCGCTATAACAGCGCAAATCCAGAAAG
CAAAATTGCCCACGCATGTGGAAATCGATGCAAAGCGTTACGGTTTGGAAAGGGATTCTGTGATATTGCTTGAACAAATA
CGCACAATCGATAAACAACGCTTAACGGATAAGATTACGCATCTTGATGAAGAAGTCATGGAGAAAATTGACGACGCCCT
GCAAATCAGCCTGGGACTGGTCGAATTTTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10633.19 Da        Isoelectric Point: 5.2204

>AT49500 WP_014096808.1 NZ_CP009709:c1927862-1927584 [Bacillus coagulans DSM 1 = ATCC 7050]
VSEPISTEILVRLPQQLLTELDGFAETENMNRNELIYRATKMYLKERKKRQIRETMRRGYMEMAKINLAIASEAIQAEYE
AEHTVESLVSGG

Download         Length: 279 bp

>AT49500 NZ_CP009709:c1927862-1927584 [Bacillus coagulans DSM 1 = ATCC 7050]
GTGTCTGAACCTATTTCAACAGAAATTTTAGTCCGCTTGCCGCAGCAGCTGTTGACGGAATTGGACGGATTTGCTGAAAC
TGAAAATATGAATCGCAATGAGTTAATTTACCGTGCCACAAAAATGTATTTAAAAGAACGGAAAAAACGCCAAATCCGCG
AAACGATGAGGCGAGGCTACATGGAAATGGCAAAAATTAATTTGGCGATTGCCTCCGAAGCGATCCAGGCTGAATACGAA
GCAGAACATACAGTTGAAAGCCTAGTAAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B4BYJ0

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4BX66

References