Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 245300..245942 Replicon chromosome
Accession NZ_CP009692
Organism Bacillus mycoides strain ATCC 6462

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BG05_RS03055 Protein ID WP_000635965.1
Coordinates 245300..245650 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BG05_RS03060 Protein ID WP_000004570.1
Coordinates 245655..245942 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG05_RS03045 242223..242678 - 456 WP_016095131.1 SprT family protein -
BG05_RS30480 242889..243005 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BG05_RS03050 243063..245231 - 2169 WP_002124824.1 RNA-binding transcriptional accessory protein -
BG05_RS03055 245300..245650 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BG05_RS03060 245655..245942 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BG05_RS03065 246254..247423 - 1170 WP_002124821.1 alanine racemase -
BG05_RS03070 247541..248491 - 951 WP_033734626.1 outer membrane lipoprotein carrier protein LolA -
BG05_RS03075 248648..249007 - 360 WP_002009969.1 holo-ACP synthase -
BG05_RS29110 249100..249648 + 549 WP_003186998.1 rhomboid family intramembrane serine protease -
BG05_RS03085 249815..250777 - 963 WP_002009966.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T49459 WP_000635965.1 NZ_CP009692:c245650-245300 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T49459 NZ_CP009692:c245650-245300 [Bacillus mycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGGGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTGGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT49459 WP_000004570.1 NZ_CP009692:c245942-245655 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT49459 NZ_CP009692:c245942-245655 [Bacillus mycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References