Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 3509767..3510394 Replicon chromosome
Accession NZ_CP009687
Organism Clostridium aceticum strain DSM 1496

Toxin (Protein)


Gene name pemK Uniprot ID A0A0D8IC31
Locus tag CACET_RS16155 Protein ID WP_044824194.1
Coordinates 3509767..3510117 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1D9FHE6
Locus tag CACET_RS16160 Protein ID WP_044824195.1
Coordinates 3510119..3510394 (-) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CACET_RS16135 3504862..3505584 - 723 WP_044824191.1 WecB/TagA/CpsF family glycosyltransferase -
CACET_RS16140 3505581..3506678 - 1098 WP_044824192.1 polysaccharide pyruvyl transferase CsaB -
CACET_RS16145 3506726..3508915 - 2190 WP_044824193.1 hypothetical protein -
CACET_RS16150 3509120..3509611 - 492 WP_052661304.1 hypothetical protein -
CACET_RS16155 3509767..3510117 - 351 WP_044824194.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CACET_RS16160 3510119..3510394 - 276 WP_044824195.1 ribbon-helix-helix protein, CopG family Antitoxin
CACET_RS16165 3510548..3511720 - 1173 WP_044824196.1 alanine racemase -
CACET_RS16170 3511758..3512387 - 630 WP_044824197.1 outer membrane lipoprotein carrier protein LolA -
CACET_RS16175 3512421..3512789 - 369 WP_044824344.1 holo-ACP synthase -
CACET_RS16180 3512811..3513434 - 624 WP_044824198.1 hypothetical protein -
CACET_RS16185 3513878..3514867 + 990 WP_044824199.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(6-43)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12792.83 Da        Isoelectric Point: 5.1457

>T49458 WP_044824194.1 NZ_CP009687:c3510117-3509767 [Clostridium aceticum]
MVVKRGDIYYADLSPVIGSEQGGTRPVLVVQNDIGNRYSPTVIIAAITSQINKAKLPTHVEIAASDYGLTKDSVILLEQI
RTIDKRRLEEKIGHLDDEMMIKVSEALLISLGLVDF

Download         Length: 351 bp

>T49458 NZ_CP009687:c3510117-3509767 [Clostridium aceticum]
TTGGTTGTAAAAAGAGGCGACATATATTATGCAGACTTAAGCCCTGTTATAGGATCTGAGCAGGGAGGAACTAGACCTGT
TTTAGTTGTACAAAATGATATAGGTAATCGGTATAGTCCTACAGTGATTATTGCTGCTATTACATCTCAAATCAACAAAG
CAAAACTCCCTACCCATGTAGAAATTGCGGCCTCAGATTATGGATTAACAAAGGATTCAGTCATATTATTGGAGCAAATT
AGAACCATTGATAAGAGAAGACTAGAAGAAAAAATAGGCCACTTAGATGATGAAATGATGATTAAGGTGAGTGAAGCACT
TTTAATTAGCCTAGGATTAGTGGACTTCTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10539.18 Da        Isoelectric Point: 4.5214

>AT49458 WP_044824195.1 NZ_CP009687:c3510394-3510119 [Clostridium aceticum]
MADSKRIMISLPESLLKEVDYIVSMEETNRSEFVREAMKLYIREKNKVKLREKMKKGYQEMASINLTLAEVGLSLDVSSL
EDYEAEIAECE

Download         Length: 276 bp

>AT49458 NZ_CP009687:c3510394-3510119 [Clostridium aceticum]
ATGGCTGATTCTAAAAGGATAATGATTAGTCTTCCTGAAAGTCTTCTTAAAGAAGTGGATTATATAGTTTCTATGGAAGA
AACAAATAGAAGTGAATTTGTCAGGGAAGCCATGAAACTATATATAAGGGAAAAAAACAAAGTCAAATTAAGAGAGAAAA
TGAAAAAGGGCTATCAAGAAATGGCTTCAATAAATTTAACATTGGCGGAGGTAGGGTTAAGTCTAGATGTATCCTCCCTA
GAAGATTATGAGGCAGAAATAGCGGAGTGTGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8IC31

Antitoxin

Source ID Structure
AlphaFold DB A0A1D9FHE6

References