Detailed information of TA system TA049453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3803051..3803865
• Replicon: chromosome
• Accession: NZ_CP009685
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: EO53_RS18880
• Protein ID: WP_001054376.1
• Coordinates: 3803051..3803308 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: EO53_RS18885
• Protein ID: WP_001309181.1
• Coordinates: 3803320..3803865 (+)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO53_RS18855	3798339..3799445	+	1107	WP_001309184.1	N-acetylneuraminate epimerase	-
EO53_RS18860	3799510..3800490	+	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
EO53_RS18865	3801073..3802313	-	1241	Protein_3593	helicase YjhR	-
EO53_RS25925	3802429..3802674	+	246	Protein_3594	GNAT family N-acetyltransferase	-
EO53_RS18880	3803051..3803308	+	258	WP_001054376.1	hypothetical protein	Toxin
EO53_RS18885	3803320..3803865	+	546	WP_001309181.1	N-acetyltransferase	Antitoxin
EO53_RS18890	3803921..3804667	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
EO53_RS25455	3804836..3805054	+	219	Protein_3598	hypothetical protein	-
EO53_RS26115	3805092..3805208	+	117	Protein_3599	VOC family protein	-
EO53_RS18895	3805453..3806574	+	1122	WP_000010829.1	M42 family metallopeptidase	-
EO53_RS18900	3806571..3806849	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
EO53_RS18905	3806861..3808174	+	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimI / fimA / fimE / fimB	3792837..3812089	19252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T49453 WP_001054376.1 NZ_CP009685:3803051-3803308 [Escherichia coli str. K-12 substr. MG1655]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T49453 NZ_CP009685:3803051-3803308 [Escherichia coli str. K-12 substr. MG1655]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT49453 WP_001309181.1 NZ_CP009685:3803320-3803865 [Escherichia coli str. K-12 substr. MG1655]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT49453 NZ_CP009685:3803320-3803865 [Escherichia coli str. K-12 substr. MG1655]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8