Detailed information of TA system TA049448

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3431452..3432131
• Replicon: chromosome
• Accession: NZ_CP009685
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: EO53_RS17085
• Protein ID: WP_000854672.1
• Coordinates: 3431790..3432131 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: EO53_RS17080
• Protein ID: WP_000070395.1
• Coordinates: 3431452..3431769 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO53_RS17040	3426499..3427362	+	864	WP_001065553.1	GTPase family protein	-
EO53_RS17045	3427454..3428275	+	822	WP_000197389.1	DUF945 domain-containing protein	-
EO53_RS25875	3428492..3428683	+	192	Protein_3247	DeoR family transcriptional regulator	-
EO53_RS25880	3428679..3428906	+	228	WP_001548158.1	hypothetical protein	-
EO53_RS25885	3428906..3429349	+	444	WP_000824223.1	hypothetical protein	-
EO53_RS17060	3429372..3429839	+	468	WP_001547765.1	hypothetical protein	-
EO53_RS17065	3429916..3430155	+	240	WP_000194654.1	DUF905 domain-containing protein	-
EO53_RS17070	3430253..3430711	+	459	WP_000211838.1	antirestriction protein	-
EO53_RS17075	3430727..3431203	+	477	WP_000811693.1	RadC family protein	-
EO53_RS24500	3431212..3431433	+	222	WP_000691994.1	DUF987 domain-containing protein	-
EO53_RS17080	3431452..3431769	+	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
EO53_RS17085	3431790..3432131	+	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
EO53_RS17095	3432703..3433956	-	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
EO53_RS17100	3433968..3435071	-	1104	WP_001285288.1	glutamate 5-kinase	-
EO53_RS17105	3435359..3436414	+	1056	WP_000749863.1	phosphoporin PhoE	-
EO53_RS17110	3436453..3436782	-	330	Protein_3260	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	3398438..3473844	75406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T49448 WP_000854672.1 NZ_CP009685:3431790-3432131 [Escherichia coli str. K-12 substr. MG1655]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T49448 NZ_CP009685:3431790-3432131 [Escherichia coli str. K-12 substr. MG1655]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT49448 WP_000070395.1 NZ_CP009685:3431452-3431769 [Escherichia coli str. K-12 substr. MG1655]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT49448 NZ_CP009685:3431452-3431769 [Escherichia coli str. K-12 substr. MG1655]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684