Detailed information of TA system TA049446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2185356..2185994
• Replicon: chromosome
• Accession: NZ_CP009685
• Organism: Escherichia coli str. K-12 substr. MG1655 strain K-12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EO53_RS10810
• Protein ID: WP_000813794.1
• Coordinates: 2185818..2185994 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EO53_RS10805
• Protein ID: WP_001270286.1
• Coordinates: 2185356..2185772 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO53_RS10785	2180508..2181449	-	942	WP_001251304.1	ABC transporter permease	-
EO53_RS10790	2181450..2182463	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
EO53_RS10795	2182481..2183626	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
EO53_RS10800	2183871..2185277	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
EO53_RS10805	2185356..2185772	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EO53_RS10810	2185818..2185994	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EO53_RS10815	2186216..2186446	+	231	WP_000494244.1	YncJ family protein	-
EO53_RS10820	2186538..2188499	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EO53_RS10825	2188572..2189108	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
EO53_RS10830	2189161..2190375	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T49446 WP_000813794.1 NZ_CP009685:c2185994-2185818 [Escherichia coli str. K-12 substr. MG1655]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T49446 NZ_CP009685:c2185994-2185818 [Escherichia coli str. K-12 substr. MG1655]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT49446 WP_001270286.1 NZ_CP009685:c2185772-2185356 [Escherichia coli str. K-12 substr. MG1655]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT49446 NZ_CP009685:c2185772-2185356 [Escherichia coli str. K-12 substr. MG1655]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB