Detailed information of TA system TA049397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3969188..3969828
• Replicon: chromosome
• Accession: NZ_CP009678
• Organism: Pectobacterium odoriferum strain BC S7

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: BCS7_RS18160
• Protein ID: WP_103165178.1
• Coordinates: 3969188..3969604 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A094RPM0
• Locus tag: BCS7_RS18165
• Protein ID: WP_039490829.1
• Coordinates: 3969601..3969828 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCS7_RS18140	3964727..3965710	-	984	WP_044208614.1	quinone oxidoreductase	-
BCS7_RS18145	3965958..3967364	+	1407	WP_039490832.1	replicative DNA helicase	-
BCS7_RS18150	3967406..3968488	+	1083	WP_039490831.1	alanine racemase	-
BCS7_RS18155	3968481..3969119	+	639	WP_103165179.1	hypothetical protein	-
BCS7_RS18160	3969188..3969604	-	417	WP_103165178.1	type II toxin-antitoxin system VapC family toxin	Toxin
BCS7_RS18165	3969601..3969828	-	228	WP_039490829.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BCS7_RS18170	3970801..3971280	+	480	WP_010295086.1	Lrp/AsnC family transcriptional regulator	-
BCS7_RS18175	3971531..3972145	+	615	WP_039490827.1	YitT family protein	-
BCS7_RS18180	3972210..3973403	+	1194	WP_103165177.1	aspartate/tyrosine/aromatic aminotransferase	-
BCS7_RS18185	3973572..3974075	+	504	WP_044208625.1	M48 family metallopeptidase	-
BCS7_RS18190	3974194..3974673	+	480	WP_010295094.1	Hcp family type VI secretion system effector	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15250.86 Da
• Isoelectric Point: 8.5225

>T49397 WP_103165178.1 NZ_CP009678:c3969604-3969188 [Pectobacterium odoriferum]
VSRLFMLDTNICSFIMREHPAEMIEKLQQCTLRRDRIVVSAITYAEMRFGVVGKKASPRHMQLVDAFCRRLDAVLPWDCA
AVDATTKIKIALAAAGTPIGPNDTAIAGHAISAKAILVTNNLREFERVPGLTLEDWGK

Nucleotide

• Length: 417 bp

>T49397 NZ_CP009678:c3969604-3969188 [Pectobacterium odoriferum]
GTGAGTCGTTTGTTCATGCTGGATACCAATATCTGTTCCTTCATTATGCGTGAACACCCTGCGGAGATGATTGAAAAGTT
GCAGCAGTGCACGCTGCGGCGTGATCGGATTGTGGTCTCTGCCATCACTTACGCTGAAATGCGTTTCGGGGTGGTTGGCA
AAAAAGCCTCGCCACGTCATATGCAACTCGTTGATGCTTTCTGTCGCCGACTTGATGCGGTGTTACCTTGGGATTGCGCC
GCAGTAGACGCTACGACGAAAATTAAAATCGCGCTAGCCGCAGCAGGCACGCCTATTGGCCCGAATGATACGGCCATCGC
AGGTCATGCCATCAGTGCTAAGGCCATATTGGTCACCAATAATCTGAGAGAATTTGAGCGAGTACCGGGGCTGACGTTGG
AAGATTGGGGTAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8861.99 Da
• Isoelectric Point: 4.5098

>AT49397 WP_039490829.1 NZ_CP009678:c3969828-3969601 [Pectobacterium odoriferum]
MRIVSVFKNGNNRAIRLPRDMDFEGVNELEIVREGDTIILRPVKPNWHSFLQEDRADADFMTERQDVVEEGRFEL

Nucleotide

• Length: 228 bp

>AT49397 NZ_CP009678:c3969828-3969601 [Pectobacterium odoriferum]
ATGCGTATTGTTTCGGTATTTAAAAACGGTAACAACCGTGCCATTCGTTTGCCACGTGATATGGACTTTGAAGGCGTCAA
CGAACTGGAGATTGTGCGTGAGGGAGATACCATCATACTGCGTCCGGTCAAACCCAATTGGCATTCGTTTTTGCAAGAAG
ATCGCGCTGACGCTGACTTTATGACAGAACGCCAGGATGTGGTCGAAGAGGGGAGATTCGAACTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A094RPM0