Detailed information of TA system TA049386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 5115077..5116068
• Replicon: chromosome
• Accession: NZ_CP009651
• Organism: Bacillus pseudomycoides strain BTZ

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: C3BKJ5
• Locus tag: BG07_RS28450
• Protein ID: WP_006094848.1
• Coordinates: 5115328..5116068 (-)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: BG07_RS28445
• Protein ID: WP_018780469.1
• Coordinates: 5115077..5115205 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG07_RS28420	5110945..5111196	-	252	WP_018780473.1	hypothetical protein	-
BG07_RS28425	5111502..5112353	-	852	WP_018780472.1	metallophosphoesterase	-
BG07_RS28430	5112657..5114123	-	1467	WP_018780471.1	aminoacyl-histidine dipeptidase	-
BG07_RS28435	5114465..5114806	+	342	WP_051977368.1	GNAT family N-acetyltransferase	-
BG07_RS28440	5114821..5114997	-	177	WP_003197743.1	hypothetical protein	-
BG07_RS28445	5115077..5115205	-	129	WP_018780469.1	hypothetical protein	Antitoxin
BG07_RS28450	5115328..5116068	-	741	WP_006094848.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
BG07_RS28455	5116266..5117000	-	735	WP_040119579.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
BG07_RS28460	5117160..5117636	-	477	WP_006094850.1	MarR family winged helix-turn-helix transcriptional regulator	-
BG07_RS28465	5117822..5118232	-	411	WP_018780467.1	VOC family protein	-
BG07_RS28470	5118550..5119806	-	1257	WP_018780466.1	MFS transporter	-
BG07_RS28475	5119874..5120509	-	636	WP_006094853.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (11-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28424.14 Da
• Isoelectric Point: 6.7286

>T49386 WP_006094848.1 NZ_CP009651:c5116068-5115328 [Bacillus pseudomycoides]
MTVSNIRVGLFLLVLVFLFLIFFYWKNEELYEEKKQLIRKTWYGVFITSVTMYFMMKGIDLTLWKNILMFVAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAAFGTMEWQTETEYAQSLNVFLDTYAEKIGAK
IVVFVTEGELNTNFRGIRSQFSITVPVEHIEQLNEQKAVQVENVGIIPAKIVRDVCIVIDGQKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T49386 NZ_CP009651:c5116068-5115328 [Bacillus pseudomycoides]
TTGACGGTCTCTAACATTCGAGTTGGTTTGTTTCTTCTCGTATTAGTCTTTTTATTTCTTATTTTCTTTTATTGGAAAAA
TGAAGAATTGTACGAAGAAAAAAAGCAACTTATTCGAAAAACATGGTATGGTGTGTTTATTACATCAGTCACAATGTATT
TCATGATGAAAGGAATTGATTTAACCCTCTGGAAAAATATTTTAATGTTCGTTGCAATGGTGATTTTTGTTGATATTGCA
TTTATTTTAACTCCAAACATTTCTGAAATATGGGGTGCAAAATTTAGTGATATCGGAAAGACCGTTCAATCTATTAAACG
ATCATTAATTGCTTCGAAAGCACGAGGTGAGATCTACACAACAATTATTCAAAATGTAAACCCAGCAGCATTTGGTACGA
TGGAGTGGCAAACTGAAACAGAATATGCACAAAGTTTAAATGTATTTCTTGATACGTATGCAGAAAAAATTGGGGCGAAG
ATTGTCGTATTTGTGACAGAAGGTGAATTAAATACGAATTTTCGTGGTATCCGTTCTCAATTTAGTATTACAGTTCCTGT
GGAACATATAGAACAATTAAATGAACAGAAAGCAGTGCAAGTTGAAAATGTTGGTATCATACCAGCCAAAATAGTAAGAG
ATGTTTGTATTGTTATTGATGGTCAAAAGAATAACCTTCAAGACCGGGATTTTGAAAATGTATATAACTTAACAATTCAT
CATAGTTATTTTAGTAAGTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4928.37 Da
• Isoelectric Point: 4.0236

>AT49386 WP_018780469.1 NZ_CP009651:c5115205-5115077 [Bacillus pseudomycoides]
VEKEYGSEFFKEEAEDTTDFSLIKGDTMQQVENLEEELKRKG

Nucleotide

• Length: 129 bp

>AT49386 NZ_CP009651:c5115205-5115077 [Bacillus pseudomycoides]
GTGGAAAAAGAATATGGTAGTGAATTTTTCAAAGAAGAAGCTGAAGATACAACAGATTTCTCTTTAATAAAAGGAGATAC
AATGCAGCAAGTAGAAAATTTAGAAGAAGAATTAAAAAGAAAAGGATAA

Structures

Toxin

• AlphaFold DB: C3BKJ5