Detailed information of TA system TA049385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2141069..2141711
• Replicon: chromosome
• Accession: NZ_CP009651
• Organism: Bacillus pseudomycoides strain BTZ

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: R8PUN4
• Locus tag: BG07_RS12710
• Protein ID: WP_003194532.1
• Coordinates: 2141069..2141419 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: R8PTJ6
• Locus tag: BG07_RS12715
• Protein ID: WP_000004571.1
• Coordinates: 2141424..2141711 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG07_RS12695	2137869..2138354	-	486	WP_018767122.1	SprT family protein	-
BG07_RS31120	2138609..2138725	+	117	WP_017154227.1	cortex morphogenetic protein CmpA	-
BG07_RS12705	2138835..2141003	-	2169	WP_018782023.1	RNA-binding transcriptional accessory protein	-
BG07_RS12710	2141069..2141419	-	351	WP_003194532.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BG07_RS12715	2141424..2141711	-	288	WP_000004571.1	antitoxin EndoAI	Antitoxin
BG07_RS12720	2141960..2143129	-	1170	WP_040119081.1	alanine racemase	-
BG07_RS12725	2143396..2144343	-	948	WP_029427332.1	outer membrane lipoprotein carrier protein LolA	-
BG07_RS12730	2144500..2144859	-	360	WP_006093158.1	holo-ACP synthase	-
BG07_RS12735	2144952..2145512	+	561	WP_042511364.1	rhomboid family intramembrane serine protease	-
BG07_RS12740	2145516..2146478	-	963	WP_018782019.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12980.10 Da
• Isoelectric Point: 5.7168

>T49385 WP_003194532.1 NZ_CP009651:c2141419-2141069 [Bacillus pseudomycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEMMMSRVDEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T49385 NZ_CP009651:c2141419-2141069 [Bacillus pseudomycoides]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCGACTGTGATTGTAGCGGCTATTACTGCACAGATTCAGAAAG
CAAAATTGCCCACACATGTGGAAATCGATGCAAAAAAGTACGGATTTGAGAGAGATTCTGTTATCTTGCTCGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAGATGATGATGAGTCGTGTAGACGAAGCTTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10748.30 Da
• Isoelectric Point: 8.9605

>AT49385 WP_000004571.1 NZ_CP009651:c2141711-2141424 [Bacillus pseudomycoides]
VSESSVTTEIVVRLPKQVVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Nucleotide

• Length: 288 bp

>AT49385 NZ_CP009651:c2141711-2141424 [Bacillus pseudomycoides]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAGTGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Structures

Toxin

• AlphaFold DB: R8PUN4

Antitoxin

• AlphaFold DB: R8PTJ6