Detailed information of TA system TA049378

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3024516..3025170
• Replicon: chromosome
• Accession: NZ_CP009644
• Organism: Escherichia coli ER2796 strain K-12 ER2796

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: ER2796_RS14990
• Protein ID: WP_000244777.1
• Coordinates: 3024516..3024923 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: ER2796_RS14995
• Protein ID: WP_046377553.1
• Coordinates: 3024940..3025170 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER2796_RS14970	3020473..3022206	-	1734	WP_000813200.1	single-stranded-DNA-specific exonuclease RecJ	-
ER2796_RS14975	3022212..3022922	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
ER2796_RS14980	3022947..3023843	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
ER2796_RS14985	3023955..3024476	+	522	WP_001055874.1	flavodoxin FldB	-
ER2796_RS14990	3024516..3024923	-	408	WP_000244777.1	toxin CptA	Toxin
ER2796_RS14995	3024940..3025170	-	231	WP_046377553.1	FAD assembly factor SdhE	Antitoxin
ER2796_RS15000	3025413..3026393	+	981	WP_000886062.1	tRNA-modifying protein YgfZ	-
ER2796_RS15005	3026589..3027248	-	660	WP_000250274.1	hemolysin III family protein	-
ER2796_RS15010	3027412..3027723	-	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
ER2796_RS15015	3027768..3029201	+	1434	WP_001336277.1	6-phospho-beta-glucosidase BglA	-
ER2796_RS15020	3029258..3030001	-	744	WP_000951964.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-76)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T49378 WP_000244777.1 NZ_CP009644:c3024923-3024516 [Escherichia coli ER2796]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T49378 NZ_CP009644:c3024923-3024516 [Escherichia coli ER2796]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9168.56 Da
• Isoelectric Point: 4.6388

>AT49378 WP_046377553.1 NZ_CP009644:c3025170-3024940 [Escherichia coli ER2796]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLI

Nucleotide

• Length: 231 bp

>AT49378 NZ_CP009644:c3025170-3024940 [Escherichia coli ER2796]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTTAG

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7