Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2067257..2068088 Replicon chromosome
Accession NZ_CP009644
Organism Escherichia coli ER2796 strain K-12 ER2796

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag ER2796_RS10425 Protein ID WP_000854814.1
Coordinates 2067714..2068088 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag ER2796_RS10420 Protein ID WP_001285584.1
Coordinates 2067257..2067625 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ER2796_RS10405 2064924..2066456 + 1533 WP_001350525.1 hypothetical protein -
ER2796_RS24855 2066453..2066899 + 447 WP_000187523.1 RadC family protein -
ER2796_RS10415 2066962..2067183 + 222 WP_000692323.1 DUF987 domain-containing protein -
ER2796_RS10420 2067257..2067625 + 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
ER2796_RS10425 2067714..2068088 + 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
ER2796_RS10430 2068085..2068279 + 195 WP_000988600.1 hypothetical protein -
ER2796_RS25625 2068292..2068405 + 114 WP_001161660.1 DUF957 domain-containing protein -
ER2796_RS23675 2068694..2068822 - 129 Protein_1990 transposase domain-containing protein -
ER2796_RS10440 2068894..2069076 + 183 WP_001016348.1 hypothetical protein -
ER2796_RS10445 2069177..2069506 - 330 WP_000450409.1 DUF496 family protein -
ER2796_RS10450 2069678..2070736 - 1059 WP_001200891.1 FUSC family protein -
ER2796_RS10455 2070934..2071407 - 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
ER2796_RS10460 2071526..2072692 - 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T49373 WP_000854814.1 NZ_CP009644:2067714-2068088 [Escherichia coli ER2796]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T49373 NZ_CP009644:2067714-2068088 [Escherichia coli ER2796]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT49373 WP_001285584.1 NZ_CP009644:2067257-2067625 [Escherichia coli ER2796]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT49373 NZ_CP009644:2067257-2067625 [Escherichia coli ER2796]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References