Detailed information of TA system TA049356

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 263888..264567
• Replicon: chromosome
• Accession: NZ_CP009644
• Organism: Escherichia coli ER2796 strain K-12 ER2796

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: ER2796_RS01270
• Protein ID: WP_000854672.1
• Coordinates: 263888..264229 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: ER2796_RS01275
• Protein ID: WP_000070395.1
• Coordinates: 264250..264567 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER2796_RS01245	259165..259566	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
ER2796_RS01250	259605..260660	-	1056	WP_000749863.1	phosphoporin PhoE	-
ER2796_RS01255	260948..262051	+	1104	WP_001285288.1	glutamate 5-kinase	-
ER2796_RS01260	262063..263316	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
ER2796_RS01270	263888..264229	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
ER2796_RS01275	264250..264567	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
ER2796_RS22900	264586..264807	-	222	WP_000691994.1	DUF987 domain-containing protein	-
ER2796_RS01280	264816..265292	-	477	WP_000811693.1	RadC family protein	-
ER2796_RS01285	265308..265766	-	459	WP_000211838.1	antirestriction protein	-
ER2796_RS01290	265864..266103	-	240	WP_000194654.1	DUF905 domain-containing protein	-
ER2796_RS01295	266180..266647	-	468	WP_001547765.1	hypothetical protein	-
ER2796_RS25110	266670..267113	-	444	WP_000824223.1	hypothetical protein	-
ER2796_RS25115	267113..267340	-	228	WP_001548158.1	hypothetical protein	-
ER2796_RS25120	267336..267527	-	192	Protein_250	DeoR family transcriptional regulator	-
ER2796_RS01310	267744..268565	-	822	WP_000197389.1	DUF945 domain-containing protein	-
ER2796_RS01315	268657..269520	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T49356 WP_000854672.1 NZ_CP009644:c264229-263888 [Escherichia coli ER2796]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T49356 NZ_CP009644:c264229-263888 [Escherichia coli ER2796]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT49356 WP_000070395.1 NZ_CP009644:c264567-264250 [Escherichia coli ER2796]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT49356 NZ_CP009644:c264567-264250 [Escherichia coli ER2796]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684