49351

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4260606..4261248 Replicon chromosome
Accession NZ_CP009641
Organism Bacillus cereus 03BB108

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag AK40_RS25995 Protein ID WP_000635963.1
Coordinates 4260898..4261248 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag AK40_RS25990 Protein ID WP_000004570.1
Coordinates 4260606..4260893 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AK40_RS25965 4255922..4256884 + 963 WP_000961158.1 UV DNA damage repair endonuclease UvsE -
AK40_RS25970 4256877..4257449 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
AK40_RS25975 4257542..4257901 + 360 WP_000635040.1 holo-ACP synthase -
AK40_RS25980 4258058..4259008 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
AK40_RS25985 4259127..4260296 + 1170 WP_000390600.1 alanine racemase -
AK40_RS25990 4260606..4260893 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
AK40_RS25995 4260898..4261248 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AK40_RS26000 4261316..4263484 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
AK40_RS26005 4263542..4263658 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
AK40_RS26010 4263854..4264312 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T49351 WP_000635963.1 NZ_CP009641:4260898-4261248 [Bacillus cereus 03BB108]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T49351 NZ_CP009641:4260898-4261248 [Bacillus cereus 03BB108]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT49351 WP_000004570.1 NZ_CP009641:4260606-4260893 [Bacillus cereus 03BB108]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT49351 NZ_CP009641:4260606-4260893 [Bacillus cereus 03BB108]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References