49340

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2383806..2384448 Replicon chromosome
Accession NZ_CP009628
Organism Bacillus cereus ATCC 4342

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BF35_RS12335 Protein ID WP_000635963.1
Coordinates 2383806..2384156 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BF35_RS12340 Protein ID WP_000004570.1
Coordinates 2384161..2384448 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BF35_RS12320 2380742..2381200 - 459 WP_000344256.1 SprT family protein -
BF35_RS12325 2381396..2381512 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BF35_RS12330 2381570..2383738 - 2169 WP_000426233.1 RNA-binding transcriptional accessory protein -
BF35_RS12335 2383806..2384156 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BF35_RS12340 2384161..2384448 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BF35_RS12345 2384758..2385927 - 1170 WP_000390607.1 alanine racemase -
BF35_RS12350 2386046..2386996 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BF35_RS12355 2387153..2387512 - 360 WP_000635043.1 holo-ACP synthase -
BF35_RS12360 2387605..2388177 + 573 WP_000906921.1 rhomboid family intramembrane serine protease -
BF35_RS12365 2388170..2389132 - 963 WP_000961159.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T49340 WP_000635963.1 NZ_CP009628:c2384156-2383806 [Bacillus cereus ATCC 4342]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T49340 NZ_CP009628:c2384156-2383806 [Bacillus cereus ATCC 4342]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT49340 WP_000004570.1 NZ_CP009628:c2384448-2384161 [Bacillus cereus ATCC 4342]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT49340 NZ_CP009628:c2384448-2384161 [Bacillus cereus ATCC 4342]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References