Detailed information of TA system TA049337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF-doc
• Location: 10094..10698
• Replicon: plasmid unamed
• Accession: NZ_CP009624
• Organism: Staphylococcus agnetis strain 908

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: L7PFJ6
• Locus tag: EP23_RS24400
• Protein ID: WP_001022435.1
• Coordinates: 10094..10432 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: L7PH55
• Locus tag: EP23_RS24405
• Protein ID: WP_001129937.1
• Coordinates: 10429..10698 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP23_RS24365	5133..5633	+	501	WP_060552539.1	hypothetical protein	-
EP23_RS24370	5783..6052	+	270	WP_060552540.1	hypothetical protein	-
EP23_RS24375	6633..6890	+	258	WP_060552541.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EP23_RS24380	6890..7156	+	267	WP_060552542.1	Txe/YoeB family addiction module toxin	-
EP23_RS24385	7587..8141	+	555	WP_001062591.1	recombinase family protein	-
EP23_RS24390	8152..8400	+	249	WP_015379476.1	helix-turn-helix transcriptional regulator	-
EP23_RS24395	9236..9919	-	684	WP_000277644.1	CPBP family intramembrane metalloprotease	-
EP23_RS24400	10094..10432	-	339	WP_001022435.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EP23_RS24405	10429..10698	-	270	WP_001129937.1	hypothetical protein	Antitoxin
EP23_RS24410	10999..11592	-	594	WP_060552544.1	IS6 family transposase	-
EP23_RS24415	11845..13004	+	1160	Protein_15	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
EP23_RS24420	13308..14039	-	732	WP_060552545.1	CPBP family intramembrane metalloprotease	-
EP23_RS24425	14152..14961	-	810	WP_015379486.1	alpha/beta hydrolase	-
EP23_RS24430	15056..15568	-	513	WP_031838161.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..28987	28987
	-	flank	IS/Tn	-	-	10999..11592	593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13001.20 Da
• Isoelectric Point: 10.1988

>T49337 WP_001022435.1 NZ_CP009624:c10432-10094 [Staphylococcus agnetis]
MNIKQFDILYIDLNPTRGREKHNVRPCLVINNQMSIDGTNFVWVLPITTRGLRYPTDIQLKTKKGLVSGVIDTVQIRALD
LKARQYNYKDELQDNLKNDILKAIKTYLKPTL

Nucleotide

• Length: 339 bp

>T49337 NZ_CP009624:c10432-10094 [Staphylococcus agnetis]
ATGAACATTAAACAATTTGACATACTCTACATTGATTTAAATCCAACTCGTGGACGTGAAAAGCATAACGTAAGGCCCTG
CTTAGTGATAAATAATCAGATGAGTATTGATGGCACTAATTTTGTTTGGGTTTTACCCATAACAACTCGGGGTTTACGTT
ATCCAACAGATATCCAACTTAAAACAAAAAAAGGATTAGTATCTGGTGTGATAGACACTGTTCAAATACGCGCTTTAGAC
CTAAAAGCACGTCAATACAACTATAAAGACGAACTACAGGACAATTTAAAAAATGATATATTAAAAGCGATAAAAACTTA
TCTTAAGCCAACGTTATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10185.65 Da
• Isoelectric Point: 10.1641

>AT49337 WP_001129937.1 NZ_CP009624:c10698-10429 [Staphylococcus agnetis]
MAKSLKDLNKTSERVFKNGNSKAISLSKKTIRLANFDIGDTVEVQKMNGGLFITKKKESIEDRIKNFFQSGGKYTELEVD
WEERVGREI

Nucleotide

• Length: 270 bp

>AT49337 NZ_CP009624:c10698-10429 [Staphylococcus agnetis]
ATGGCTAAATCATTAAAAGACTTAAACAAGACTTCGGAACGAGTTTTTAAAAATGGAAACAGTAAAGCAATCAGTTTAAG
TAAAAAAACTATCAGGTTAGCTAATTTTGATATAGGAGATACAGTTGAAGTTCAAAAAATGAATGGTGGTTTGTTCATTA
CTAAAAAAAAGGAGTCTATTGAAGATAGAATTAAAAATTTCTTTCAAAGTGGTGGAAAGTATACAGAATTAGAAGTAGAT
TGGGAAGAAAGAGTTGGCCGAGAAATATGA

Structures

Toxin

• PDB: 7EWJ
• PDB: 7EWI

Antitoxin

• AlphaFold DB: L7PH55