Detailed information of TA system TA049313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 513341..513878
• Replicon: chromosome
• Accession: NZ_CP009602
• Organism: Burkholderia thailandensis 2002721643

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: BG87_RS19295
• Protein ID: WP_004549731.1
• Coordinates: 513341..513610 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q2T6G1
• Locus tag: BG87_RS19300
• Protein ID: WP_011401115.1
• Coordinates: 513594..513878 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG87_RS19255	508718..509539	+	822	WP_009896491.1	hypothetical protein	-
BG87_RS19260	509536..509796	+	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
BG87_RS19265	509950..510942	+	993	WP_015967393.1	hypothetical protein	-
BG87_RS19270	510939..511490	+	552	WP_011401112.1	DUF1064 domain-containing protein	-
BG87_RS19275	511487..511843	+	357	WP_009896497.1	hypothetical protein	-
BG87_RS31595	512283..512615	+	333	WP_011401114.1	hypothetical protein	-
BG87_RS19285	512591..512851	+	261	WP_009896498.1	hypothetical protein	-
BG87_RS19290	512848..513291	+	444	WP_009896499.1	hypothetical protein	-
BG87_RS19295	513341..513610	+	270	WP_004549731.1	BrnT family toxin	Toxin
BG87_RS19300	513594..513878	+	285	WP_011401115.1	BrnA antitoxin family protein	Antitoxin
BG87_RS19310	514509..514988	+	480	WP_025404174.1	terminase small subunit	-
BG87_RS19315	514988..516730	+	1743	WP_011401117.1	terminase large subunit	-
BG87_RS19320	516738..518033	+	1296	WP_009896509.1	phage portal protein	-
BG87_RS19325	518011..518853	+	843	WP_011401119.1	Clp protease ClpP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	493909..538552	44643

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T49313 WP_004549731.1 NZ_CP009602:513341-513610 [Burkholderia thailandensis 2002721643]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T49313 NZ_CP009602:513341-513610 [Burkholderia thailandensis 2002721643]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10669.09 Da
• Isoelectric Point: 5.8930

>AT49313 WP_011401115.1 NZ_CP009602:513594-513878 [Burkholderia thailandensis 2002721643]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLKEHQPA

Nucleotide

• Length: 285 bp

>AT49313 NZ_CP009602:513594-513878 [Burkholderia thailandensis 2002721643]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTACGACGTCGACATCGTGGACGCCTTCAAGGCAACAGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAAGGAGCACCAGCCTGCGTGA

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: Q2T6G1