Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3385210..3385852 Replicon chromosome
Accession NZ_CP009596
Organism Bacillus cereus strain 3a

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BG11_RS19310 Protein ID WP_000635963.1
Coordinates 3385210..3385560 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BG11_RS19315 Protein ID WP_000004570.1
Coordinates 3385565..3385852 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BG11_RS19295 3382146..3382604 - 459 WP_000344248.1 SprT family protein -
BG11_RS19300 3382800..3382916 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BG11_RS19305 3382974..3385142 - 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BG11_RS19310 3385210..3385560 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BG11_RS19315 3385565..3385852 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BG11_RS19320 3386162..3387331 - 1170 WP_000390600.1 alanine racemase -
BG11_RS19325 3387450..3388400 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BG11_RS19330 3388557..3388916 - 360 WP_000635040.1 holo-ACP synthase -
BG11_RS19335 3389009..3389581 + 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BG11_RS19340 3389574..3390536 - 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T49307 WP_000635963.1 NZ_CP009596:c3385560-3385210 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T49307 NZ_CP009596:c3385560-3385210 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT49307 WP_000004570.1 NZ_CP009596:c3385852-3385565 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT49307 NZ_CP009596:c3385852-3385565 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References