Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2229782..2230424 Replicon chromosome
Accession NZ_CP009590
Organism Bacillus cereus G9241

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag AQ16_RS11680 Protein ID WP_000635963.1
Coordinates 2229782..2230132 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag AQ16_RS11685 Protein ID WP_000004570.1
Coordinates 2230137..2230424 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AQ16_RS11665 2226718..2227176 - 459 WP_001975527.1 SprT family protein -
AQ16_RS11670 2227372..2227488 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
AQ16_RS11675 2227546..2229714 - 2169 WP_001975517.1 RNA-binding transcriptional accessory protein -
AQ16_RS11680 2229782..2230132 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
AQ16_RS11685 2230137..2230424 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
AQ16_RS11690 2230734..2231903 - 1170 WP_000390607.1 alanine racemase -
AQ16_RS11695 2232021..2232971 - 951 WP_033691678.1 outer membrane lipoprotein carrier protein LolA -
AQ16_RS11700 2233128..2233487 - 360 WP_000635047.1 holo-ACP synthase -
AQ16_RS11705 2233580..2234152 + 573 WP_001975497.1 rhomboid family intramembrane serine protease -
AQ16_RS11710 2234145..2235107 - 963 WP_000961159.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T49305 WP_000635963.1 NZ_CP009590:c2230132-2229782 [Bacillus cereus G9241]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T49305 NZ_CP009590:c2230132-2229782 [Bacillus cereus G9241]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT49305 WP_000004570.1 NZ_CP009590:c2230424-2230137 [Bacillus cereus G9241]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT49305 NZ_CP009590:c2230424-2230137 [Bacillus cereus G9241]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References