Detailed information of TA system TA049299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 1246252..1246789
• Replicon: chromosome
• Accession: NZ_CP009586
• Organism: Burkholderia pseudomallei strain PHLS 112

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A0F6L9W9
• Locus tag: DP45_RS22715
• Protein ID: WP_009971435.1
• Coordinates: 1246520..1246789 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP45_RS22710
• Protein ID: WP_004525420.1
• Coordinates: 1246252..1246536 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP45_RS22685	1241360..1242622	-	1263	WP_004551329.1	c-type cytochrome	-
DP45_RS22690	1242781..1244241	-	1461	WP_011326543.1	metallophosphoesterase	-
DP45_RS22695	1244488..1244604	+	117	Protein_973	IS3 family transposase	-
DP45_RS35885	1244831..1245259	-	429	Protein_974	IS110 family transposase	-
DP45_RS35890	1245222..1245605	-	384	Protein_975	DDE-type integrase/transposase/recombinase	-
DP45_RS22710	1246252..1246536	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP45_RS22715	1246520..1246789	-	270	WP_009971435.1	BrnT family toxin	Toxin
DP45_RS22720	1247341..1247616	+	276	WP_004537565.1	bacteriophage protein Gp49	-
DP45_RS22735	1247893..1248114	+	222	WP_004538072.1	hypothetical protein	-
DP45_RS40000	1248162..1248380	+	219	WP_004552392.1	hypothetical protein	-
DP45_RS22745	1248611..1249192	-	582	WP_004542548.1	hypothetical protein	-
DP45_RS22750	1249460..1249597	+	138	WP_009971437.1	hypothetical protein	-
DP45_RS40465	1249594..1250367	+	774	Protein_983	prohibitin family protein	-
DP45_RS40470	1250365..1250793	+	429	Protein_984	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1242781..1262152	19371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10143.43 Da
• Isoelectric Point: 5.6831

>T49299 WP_009971435.1 NZ_CP009586:c1246789-1246520 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVVGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T49299 NZ_CP009586:c1246789-1246520 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGGTCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT49299 WP_004525420.1 NZ_CP009586:c1246536-1246252 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT49299 NZ_CP009586:c1246536-1246252 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGTTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTACGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0F6L9W9

Antitoxin

• AlphaFold DB: A0A0F6L9S8