Detailed information of TA system TA049296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1986509..1987550
• Replicon: chromosome
• Accession: NZ_CP009585
• Organism: Burkholderia pseudomallei strain PHLS 112

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: DP45_RS09255
• Protein ID: WP_009972574.1
• Coordinates: 1986975..1987550 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A0E1SLI7
• Locus tag: DP45_RS09250
• Protein ID: WP_004540053.1
• Coordinates: 1986509..1986970 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP45_RS09230	1981613..1982212	-	600	WP_076871524.1	restriction endonuclease fold toxin 5 domain-containing protein	-
DP45_RS09240	1982554..1983060	-	507	WP_076901090.1	DUF4123 domain-containing protein	-
DP45_RS09245	1983057..1983479	-	423	WP_012729878.1	PAAR domain-containing protein	-
DP45_RS40350	1984176..1985006	+	831	WP_198403620.1	retron St85 family effector protein	-
DP45_RS32635	1984981..1985937	+	957	WP_076886478.1	retron St85 family RNA-directed DNA polymerase	-
DP45_RS09250	1986509..1986970	+	462	WP_004540053.1	excisionase family DNA-binding protein	Antitoxin
DP45_RS09255	1986975..1987550	+	576	WP_009972574.1	PIN domain-containing protein	Toxin
DP45_RS09260	1987783..1988751	+	969	WP_139897735.1	hypothetical protein	-
DP45_RS09265	1988952..1989944	+	993	WP_009972572.1	DUF3644 domain-containing protein	-
DP45_RS32645	1990171..1990410	-	240	WP_076853220.1	DNA mismatch repair protein	-
DP45_RS09270	1991247..1991426	+	180	WP_004191077.1	hypothetical protein	-
DP45_RS09275	1991610..1991882	+	273	WP_004538129.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-114)

Antitoxin

• Helix-turn-helix domain, group 17 (83-129)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 22124.51 Da
• Isoelectric Point: 6.2954

>T49296 WP_009972574.1 NZ_CP009585:1986975-1987550 [Burkholderia pseudomallei]
MSSNFTVVYDACVLYPAPLRDLLMHLALTDLYRARWTDMIHDEWTRNLLAKRTDLKPEAIQRTRELMDMHVRDAKVENFE
HLIQVIELPDPDDRHVVAAAMYAGAELIVTMNIKDFPKERVAKYNVSAQHPDDFIVDLWDLNKHKVMEAVARHRKSMKNP
PKAVEDYLDTMASQGLVQTVDALRPYAPWAI

Nucleotide

• Length: 576 bp

>T49296 NZ_CP009585:1986975-1987550 [Burkholderia pseudomallei]
ATGTCGTCGAACTTTACCGTTGTCTACGATGCGTGCGTCCTATATCCGGCTCCGTTGCGCGACTTGTTGATGCATCTCGC
GCTGACGGACCTGTACCGCGCGCGTTGGACCGATATGATTCATGACGAGTGGACAAGGAATTTGCTCGCGAAGCGGACCG
ACCTGAAACCCGAGGCGATCCAGCGCACGCGGGAACTGATGGACATGCATGTTCGCGACGCGAAAGTTGAAAACTTCGAG
CATTTGATCCAGGTGATCGAGCTGCCCGACCCTGACGACCGTCATGTCGTCGCTGCTGCCATGTATGCTGGGGCGGAACT
CATCGTCACGATGAACATCAAGGATTTCCCGAAGGAGCGGGTCGCAAAGTACAACGTGTCGGCTCAACATCCTGACGATT
TCATCGTCGACTTGTGGGACTTGAACAAGCACAAGGTGATGGAGGCGGTCGCGCGACATCGCAAGTCGATGAAGAATCCG
CCGAAAGCGGTGGAAGACTATCTCGACACGATGGCCAGCCAAGGACTCGTGCAGACGGTTGATGCGCTGCGGCCGTATGC
GCCTTGGGCTATCTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17155.41 Da
• Isoelectric Point: 5.5474

>AT49296 WP_004540053.1 NZ_CP009585:1986509-1986970 [Burkholderia pseudomallei]
MNTTVTPAALPSEHEAELARESRRNLVTVVNSHAETQQFDFKDEKGETHTVTLPTSALRLLDEILHQIGNGNAVSIIPIH
AELTTQQAADLLNVSRPYLVQLLEQGEIPFRKVNTHRRVMYLDLIQYKNKIDANRLDALAELTAQAQELGMGY

Nucleotide

• Length: 462 bp

>AT49296 NZ_CP009585:1986509-1986970 [Burkholderia pseudomallei]
ATGAACACCACTGTCACTCCTGCGGCCCTCCCGTCCGAACACGAGGCCGAGCTCGCGCGCGAGTCGCGGCGGAATTTGGT
CACGGTCGTGAACAGCCACGCCGAGACTCAGCAGTTTGATTTCAAGGACGAAAAAGGGGAGACGCATACAGTCACGCTTC
CGACCTCGGCGTTAAGGCTGCTAGACGAGATCCTCCATCAGATCGGAAACGGCAACGCGGTGTCGATCATTCCAATCCAT
GCCGAATTGACGACCCAGCAAGCCGCCGACCTTCTGAATGTCTCGCGTCCGTACCTAGTTCAACTTCTTGAGCAGGGAGA
GATCCCATTCCGGAAGGTAAATACGCATCGACGGGTGATGTATCTGGATCTGATTCAGTACAAAAACAAGATCGATGCGA
ACCGTCTCGACGCGCTGGCTGAACTGACGGCTCAGGCGCAGGAACTTGGAATGGGATATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E1SLI7