Detailed information of TA system TA049289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 325503..326047
• Replicon: chromosome
• Accession: NZ_CP009571
• Organism: Sphingomonas taxi strain ATCC 55669

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MC45_RS01420
• Protein ID: WP_038658630.1
• Coordinates: 325724..326047 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A097ECJ4
• Locus tag: MC45_RS01415
• Protein ID: WP_038658627.1
• Coordinates: 325503..325727 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MC45_RS18770	320980..321885	-	906	WP_081974283.1	NAD(P)/FAD-dependent oxidoreductase	-
MC45_RS01405	321989..322438	+	450	WP_038666180.1	Rrf2 family transcriptional regulator	-
MC45_RS18775	322688..323224	+	537	WP_081974284.1	DnaJ domain-containing protein	-
MC45_RS18785	324361..324849	+	489	Protein_286	MucR family transcriptional regulator	-
MC45_RS01410	324890..325168	-	279	Protein_287	integrase	-
MC45_RS01415	325503..325727	+	225	WP_038658627.1	antitoxin MazE family protein	Antitoxin
MC45_RS01420	325724..326047	+	324	WP_038658630.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MC45_RS19140	326055..326375	+	321	WP_156143745.1	hypothetical protein	-
MC45_RS01430	326541..327080	+	540	Protein_291	SDR family oxidoreductase	-
MC45_RS01435	327269..327901	-	633	WP_038658636.1	tyrosine-type recombinase/integrase	-
MC45_RS19145	327905..328207	-	303	WP_156143746.1	hypothetical protein	-
MC45_RS01440	328288..329208	+	921	WP_038658638.1	VOC family protein	-
MC45_RS18790	329319..330296	-	978	WP_081974286.1	bifunctional methionine sulfoxide reductase B/A protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	292605..342790	50185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-104)

Antitoxin

• Antitoxin MazE-like (7-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11384.31 Da
• Isoelectric Point: 10.1808

>T49289 WP_038658630.1 NZ_CP009571:325724-326047 [Sphingomonas taxi]
VNRGDLVTIALPGDFGKPRPALIIQSDQFEQTGTVTVLLVSGTLVDAPLIRTTIDPTPANGLRKRSQVMVDKAMSVKRDK
IGATIGHLDAETMLAVTRALAVFFAIA

Nucleotide

• Length: 324 bp

>T49289 NZ_CP009571:325724-326047 [Sphingomonas taxi]
GTGAACCGGGGGGACCTGGTGACGATCGCGCTGCCCGGTGATTTCGGCAAGCCGCGACCAGCCCTCATCATCCAGTCGGA
CCAGTTCGAACAGACCGGCACCGTCACGGTGCTGCTGGTGTCGGGTACGCTGGTGGACGCGCCCCTCATCCGCACGACGA
TCGACCCGACACCGGCCAACGGCTTGCGCAAGCGGTCGCAGGTCATGGTGGACAAGGCCATGTCGGTGAAGCGCGACAAG
ATCGGGGCGACGATCGGCCACCTCGATGCCGAAACCATGCTGGCGGTCACGCGAGCGCTTGCCGTCTTCTTCGCCATCGC
CTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8132.23 Da
• Isoelectric Point: 4.7223

>AT49289 WP_038658627.1 NZ_CP009571:325503-325727 [Sphingomonas taxi]
VPAPVTQRVQKRRDALRAAGLRPIQIWVPDTRRPGFAEECRRQALVVAAADVADHDLDAFMDAALIDLDPEGEA

Nucleotide

• Length: 225 bp

>AT49289 NZ_CP009571:325503-325727 [Sphingomonas taxi]
GTGCCGGCACCAGTGACACAACGAGTCCAGAAGCGTCGCGACGCCCTGCGCGCGGCCGGTCTGCGCCCCATCCAGATATG
GGTTCCCGATACCCGACGACCGGGGTTCGCGGAGGAATGCCGGCGACAGGCGCTCGTGGTCGCGGCGGCCGACGTGGCGG
ACCATGATCTGGACGCCTTCATGGATGCAGCCCTGATCGATCTCGACCCCGAGGGCGAGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A097ECJ4