Detailed information of TA system TA049256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 527798..528239
• Replicon: chromosome
• Accession: NZ_CP009550
• Organism: Burkholderia pseudomallei PB08298010

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: BG24_RS02175
• Protein ID: WP_009939402.1
• Coordinates: 527798..527971 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: BG24_RS02180
• Protein ID: WP_004525420.1
• Coordinates: 527955..528239 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BG24_RS41580	523888..524103	-	216	Protein_421	DDE-type integrase/transposase/recombinase	-
BG24_RS02135	524101..524874	-	774	Protein_422	prohibitin family protein	-
BG24_RS41585	524871..525020	-	150	WP_004537646.1	bacteriophage protein Gp44	-
BG24_RS02145	525279..525860	+	582	WP_004542548.1	hypothetical protein	-
BG24_RS40655	526091..526390	-	300	WP_160458000.1	hypothetical protein	-
BG24_RS02155	526357..526578	-	222	WP_004552390.1	hypothetical protein	-
BG24_RS41590	526714..526845	-	132	WP_009923452.1	bacteriophage protein Gp48	-
BG24_RS02170	526855..527130	-	276	WP_009975384.1	hypothetical protein	-
BG24_RS02175	527798..527971	+	174	WP_009939402.1	BrnT family toxin	Toxin
BG24_RS02180	527955..528239	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
BG24_RS32340	528886..529080	+	195	Protein_431	DDE-type integrase/transposase/recombinase	-
BG24_RS02190	529294..530343	+	1050	WP_028359486.1	IS110 family transposase	-
BG24_RS32345	530356..530544	+	189	Protein_433	transposase	-
BG24_RS32350	530507..530935	+	429	Protein_434	IS110 family transposase	-
BG24_RS02195	531162..531278	-	117	Protein_435	IS3 family transposase	-
BG24_RS02200	531544..533004	+	1461	WP_028358298.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ddhA	523394..602491	79097

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T49256 WP_009939402.1 NZ_CP009550:527798-527971 [Burkholderia pseudomallei PB08298010]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T49256 NZ_CP009550:527798-527971 [Burkholderia pseudomallei PB08298010]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT49256 WP_004525420.1 NZ_CP009550:527955-528239 [Burkholderia pseudomallei PB08298010]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT49256 NZ_CP009550:527955-528239 [Burkholderia pseudomallei PB08298010]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8