Detailed information of TA system TA049251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 265216..265865
• Replicon: plasmid pBTU
• Accession: NZ_CP009547
• Organism: Burkholderia sp. 2002721687

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BW21_RS01085
• Protein ID: WP_006029599.1
• Coordinates: 265216..265626 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7U4P815
• Locus tag: BW21_RS01090
• Protein ID: WP_006029598.1
• Coordinates: 265623..265865 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BW21_RS01070	262316..263044	-	729	WP_009916772.1	hypothetical protein	-
BW21_RS01075	263083..263850	-	768	WP_006029601.1	hypothetical protein	-
BW21_RS01080	264148..264606	+	459	WP_006029600.1	hypothetical protein	-
BW21_RS37375	264974..265138	-	165	WP_156436729.1	hypothetical protein	-
BW21_RS01085	265216..265626	-	411	WP_006029599.1	type II toxin-antitoxin system VapC family toxin	Toxin
BW21_RS01090	265623..265865	-	243	WP_006029598.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
BW21_RS01095	266373..267134	+	762	WP_144411849.1	hypothetical protein	-
BW21_RS01100	267333..268301	-	969	WP_043283354.1	ParB/RepB/Spo0J family partition protein	-
BW21_RS01105	268298..269506	-	1209	WP_009916766.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	bspR2 / bspI2	1..305110	305110

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14767.04 Da
• Isoelectric Point: 5.1526

>T49251 WP_006029599.1 NZ_CP009547:c265626-265216 [Burkholderia sp. 2002721687]
MTLYLLDTNILSNVIRDPRGACTARIGETPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRAELEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFARVAGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T49251 NZ_CP009547:c265626-265216 [Burkholderia sp. 2002721687]
ATGACGCTGTATCTGCTCGACACCAACATTTTGTCGAACGTGATCCGAGACCCGCGCGGCGCATGCACCGCCCGCATCGG
AGAAACACCGCCCGAACAAGTGTGCACCTCGATCATCGTGGCCGCGGAACTCCGGTTTGGCGTCTGGAAGCGCGGATCGT
CGACGCTCGCGCAGCGCGTCGAGCAACTGCTCGCGAGCTTGACCGTGTTGCCGCTGCAGCCTGACGCAGACCGTTGCTAC
GGCCGGTTGCGGGCCGAGCTCGAGAAGCAAGGCCAGTTGATCGGCGCCAACGACATGCTAATCGCCGCGCATGCGCTGGC
CGTGGACGCCGTCCTGGTCACCGACAACACGGCGGAATTTGCTCGGGTCGCGGGGCTGCCAGTCGAAAACTGGTTGCGCC
CGGCGACGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8499

>AT49251 WP_006029598.1 NZ_CP009547:c265865-265623 [Burkholderia sp. 2002721687]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDETFPDIDNLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT49251 NZ_CP009547:c265865-265623 [Burkholderia sp. 2002721687]
ATGCCAACCCATGACACCCGCCACGCGCGCCTGTTTCGCAATGGGCGCAGCCAGGCCGTGCGGATCCCGCGCGAGTTCGA
GCTACCGGTCTCGGAAGTCACTATCCACCGGGAAGGCCGGCGCTTGATTATCGAGCCCGTGGAGGCCGCGCCCCTGCGTG
AGCTGTTCGCCCAATGGACGCCGCTGGACGAAACCTTTCCCGACATCGATAACCTGCCGGCTGAACCGGTCGACATCGAA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4P815