49214

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4459938..4460578 Replicon chromosome
Accession NZ_CP009533
Organism Pseudomonas rhizosphaerae strain DSM 16299

Toxin (Protein)


Gene name vapC Uniprot ID A0A089YYN1
Locus tag LT40_RS19785 Protein ID WP_043192900.1
Coordinates 4460168..4460578 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A089ZRL0
Locus tag LT40_RS19780 Protein ID WP_043192898.1
Coordinates 4459938..4460168 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LT40_RS19765 4455315..4456184 - 870 WP_043192893.1 oxaloacetate decarboxylase -
LT40_RS19770 4456414..4457631 + 1218 WP_043192894.1 MFS transporter -
LT40_RS19775 4457628..4459679 - 2052 WP_202807717.1 EAL domain-containing protein -
LT40_RS19780 4459938..4460168 + 231 WP_043192898.1 antitoxin Antitoxin
LT40_RS19785 4460168..4460578 + 411 WP_043192900.1 tRNA(fMet)-specific endonuclease VapC Toxin
LT40_RS19790 4460651..4461751 - 1101 WP_043192902.1 redox-regulated ATPase YchF -
LT40_RS19795 4461773..4462357 - 585 WP_043192904.1 aminoacyl-tRNA hydrolase -
LT40_RS19800 4462431..4463036 - 606 WP_043192906.1 50S ribosomal protein L25/general stress protein Ctc -
LT40_RS19805 4463175..4464116 - 942 WP_043192909.1 ribose-phosphate pyrophosphokinase -
LT40_RS19815 4464277..4465122 - 846 WP_043193855.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-124)

Antitoxin

SpoVT-AbrB domain

(8-52)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 15232.76 Da        Isoelectric Point: 8.5246

>T49214 WP_043192900.1 NZ_CP009533:4460168-4460578 [Pseudomonas rhizosphaerae]
MLKFMLDTNICIFTIKNRPEHVRAAFKRSHGLMCISSVTLMELIYGAEKSLHVQRNLEVVESFAARLEVLDYDSFAAAHT
GQLRAELARLGMPIGAYDQMIAGHARSKGLILVSNNTREFMRVPGLRIEDWATPAA

Download         Length: 411 bp

>T49214 NZ_CP009533:4460168-4460578 [Pseudomonas rhizosphaerae]
ATGTTGAAGTTCATGCTCGACACCAATATCTGCATCTTCACCATCAAGAACCGTCCGGAACATGTTCGCGCTGCCTTCAA
GCGTAGTCACGGGCTGATGTGCATCAGCAGTGTAACGCTGATGGAGTTGATCTACGGGGCTGAAAAATCCCTGCACGTTC
AGCGCAATCTCGAAGTCGTGGAAAGCTTCGCCGCGCGCCTGGAAGTGCTCGATTACGATTCGTTCGCGGCCGCTCATACC
GGGCAATTGCGAGCCGAGTTGGCTCGCTTGGGCATGCCGATCGGAGCTTACGATCAGATGATCGCCGGGCATGCCCGCTC
CAAAGGCCTGATCCTGGTGAGTAACAACACCAGGGAGTTCATGCGGGTGCCTGGGCTTCGAATCGAAGATTGGGCAACAC
CGGCAGCCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8387.49 Da        Isoelectric Point: 4.5045

>AT49214 WP_043192898.1 NZ_CP009533:4459938-4460168 [Pseudomonas rhizosphaerae]
MEQGSVFLSNKSQAVRLPKALALPDGVKRVDVVAIGRTRILTPAGESWDSWFEGEAVTSDFMLDREQPAIQERDGL

Download         Length: 231 bp

>AT49214 NZ_CP009533:4459938-4460168 [Pseudomonas rhizosphaerae]
ATGGAGCAGGGCTCTGTTTTTCTGAGCAATAAAAGCCAAGCCGTTCGTCTGCCCAAGGCGCTGGCATTGCCCGATGGCGT
CAAGCGTGTCGACGTTGTTGCCATAGGTCGGACGCGAATCCTGACCCCAGCTGGCGAGTCATGGGACAGCTGGTTCGAGG
GTGAAGCTGTGACCAGCGACTTCATGCTTGACCGAGAGCAGCCAGCCATCCAAGAGCGCGACGGGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A089YYN1

Antitoxin

Source ID Structure
AlphaFold DB A0A089ZRL0

References