Detailed information of TA system TA049212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3966617..3967221
• Replicon: chromosome
• Accession: NZ_CP009533
• Organism: Pseudomonas rhizosphaerae strain DSM 16299

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LT40_RS21420
• Protein ID: WP_084139839.1
• Coordinates: 3966958..3967221 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A089YU59
• Locus tag: LT40_RS17610
• Protein ID: WP_043192393.1
• Coordinates: 3966617..3966961 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT40_RS17585	3961653..3962717	+	1065	WP_043192388.1	uroporphyrinogen decarboxylase	-
LT40_RS17590	3962826..3964094	-	1269	WP_043192389.1	MFS transporter	-
LT40_RS17595	3964299..3965069	+	771	WP_043192390.1	ParA family protein	-
LT40_RS17600	3965293..3965736	-	444	WP_043192391.1	thioredoxin TrxC	-
LT40_RS17605	3965893..3966615	+	723	WP_043192392.1	aspartate/glutamate racemase family protein	-
LT40_RS17610	3966617..3966961	-	345	WP_043192393.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LT40_RS21420	3966958..3967221	-	264	WP_084139839.1	type II toxin-antitoxin system HicA family toxin	Toxin
LT40_RS17615	3967418..3968863	-	1446	WP_043192394.1	YdiU family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algZ / algR / wbtL	3649989..3996083	346094

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (26-76)

Antitoxin

• Uncharacterised protein family HicB (56-105)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9691.29 Da
• Isoelectric Point: 10.6626

>T49212 WP_084139839.1 NZ_CP009533:c3967221-3966958 [Pseudomonas rhizosphaerae]
MNLSKLKHIDVLEAIYARPVSGTIRWSRIERLFVELGAKVQEREGSCVAVVLFGAVRVFHRPHPSPETDKAAVASIRNWL
ATNGVKP

Nucleotide

• Length: 264 bp

>T49212 NZ_CP009533:c3967221-3966958 [Pseudomonas rhizosphaerae]
ATGAACCTTTCAAAGCTCAAACACATTGACGTGCTTGAGGCGATCTACGCTCGCCCAGTATCAGGAACCATCCGATGGTC
ACGCATCGAGAGATTGTTCGTCGAATTAGGGGCCAAGGTGCAAGAGCGAGAGGGCTCCTGCGTGGCGGTCGTGCTTTTCG
GGGCCGTGCGGGTATTCCATCGTCCTCATCCGTCCCCAGAAACCGACAAGGCTGCAGTGGCAAGCATCCGCAACTGGTTG
GCAACCAACGGAGTCAAACCATGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12466.20 Da
• Isoelectric Point: 6.0728

>AT49212 WP_043192393.1 NZ_CP009533:c3966961-3966617 [Pseudomonas rhizosphaerae]
MNNIMIINGHRAVIQYDPEIGTFRGEFINLNGGADFHADSVSALKTAGAESLRVFLQVCAEEGIEPVRSYSGKFQLRLPE
ALHAQVTETAAALGVSLNQFVQRSLEHELKAVKL

Nucleotide

• Length: 345 bp

>AT49212 NZ_CP009533:c3966961-3966617 [Pseudomonas rhizosphaerae]
ATGAACAACATCATGATCATCAACGGCCATCGGGCCGTCATTCAGTACGACCCGGAGATCGGCACATTTCGCGGAGAGTT
CATTAATCTGAATGGCGGCGCGGATTTCCATGCCGACAGCGTCAGCGCATTGAAGACTGCAGGCGCCGAGTCACTGCGGG
TGTTTCTACAGGTGTGCGCTGAAGAAGGCATCGAACCTGTCAGATCCTACTCTGGGAAATTCCAGCTGCGGCTGCCTGAG
GCGCTGCACGCCCAAGTTACCGAGACAGCGGCTGCGCTGGGTGTCAGTCTTAATCAGTTCGTGCAGCGCAGCCTCGAGCA
TGAGCTCAAGGCGGTGAAGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A089YU59