Detailed information of TA system TA049211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3188069..3188733
• Replicon: chromosome
• Accession: NZ_CP009533
• Organism: Pseudomonas rhizosphaerae strain DSM 16299

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LT40_RS14120
• Protein ID: WP_043191296.1
• Coordinates: 3188069..3188473 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A089ZQZ8
• Locus tag: LT40_RS14125
• Protein ID: WP_043191304.1
• Coordinates: 3188470..3188733 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LT40_RS14105	3183336..3185279	+	1944	WP_043191287.1	CBS domain-containing protein	-
LT40_RS14110	3185276..3185986	+	711	WP_043191290.1	3'-5' exonuclease	-
LT40_RS14115	3186346..3188037	+	1692	WP_043191293.1	ABC transporter ATP-binding protein/permease	-
LT40_RS14120	3188069..3188473	-	405	WP_043191296.1	type II toxin-antitoxin system VapC family toxin	Toxin
LT40_RS14125	3188470..3188733	-	264	WP_043191304.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LT40_RS14130	3188883..3189389	-	507	WP_043193694.1	ATP-dependent zinc protease	-
LT40_RS14135	3189533..3190423	+	891	WP_043191305.1	acyltransferase	-
LT40_RS14140	3190424..3190996	-	573	WP_043191306.1	DUF2780 domain-containing protein	-
LT40_RS14145	3191320..3192519	-	1200	WP_043191307.1	formaldehyde dehydrogenase, glutathione-independent	-
LT40_RS14150	3192715..3193572	-	858	WP_043191308.1	formyltetrahydrofolate deformylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• SpoVT-AbrB domain (8-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14192.48 Da
• Isoelectric Point: 7.2917

>T49211 WP_043191296.1 NZ_CP009533:c3188473-3188069 [Pseudomonas rhizosphaerae]
MKQLMLDTNTVSHLLKGHPGVIKNLSAVPMTSVCISSITEAELLYGLAKRPSAQRLAGIVTAFLKTIDILPWGSQAAQAY
GSSRATGESRGHCLGALDGLIAAHSLSLGMTLVTSDKAFRLVEGLEIQDWCEVA

Nucleotide

• Length: 405 bp

>T49211 NZ_CP009533:c3188473-3188069 [Pseudomonas rhizosphaerae]
ATGAAGCAATTGATGCTGGACACCAATACCGTGAGCCACCTGCTCAAGGGGCATCCGGGTGTCATCAAGAACCTAAGCGC
TGTGCCGATGACATCGGTGTGCATCTCCTCCATCACCGAGGCAGAATTGCTTTATGGCTTGGCCAAGCGGCCATCTGCGC
AGCGGTTGGCAGGGATTGTCACCGCGTTCCTGAAAACCATCGATATTCTTCCTTGGGGCAGCCAGGCGGCCCAGGCCTAT
GGTTCGAGCCGGGCCACGGGCGAGAGTCGTGGGCACTGTCTGGGCGCGTTGGACGGCCTTATCGCCGCCCATAGCCTTTC
CCTCGGGATGACGCTGGTCACCAGCGACAAGGCGTTTCGCTTGGTCGAGGGGCTTGAAATACAGGACTGGTGCGAGGTCG
CGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9647.79 Da
• Isoelectric Point: 4.5457

>AT49211 WP_043191304.1 NZ_CP009533:c3188733-3188470 [Pseudomonas rhizosphaerae]
MSQTAKVFVNGRSQAVRLPAAFRFDTDEVFIRRDPETGDVILSRKPDSWNEFLALVAQGGVPDNFLDAADRAQMARGNRD
PLDGIPE

Nucleotide

• Length: 264 bp

>AT49211 NZ_CP009533:c3188733-3188470 [Pseudomonas rhizosphaerae]
ATGTCACAGACAGCAAAAGTCTTCGTAAACGGCCGAAGCCAGGCCGTGAGATTGCCTGCCGCTTTCCGATTCGACACCGA
TGAAGTCTTCATACGGCGTGATCCCGAGACCGGCGATGTGATCTTGTCCAGAAAGCCGGACTCATGGAATGAGTTTCTGG
CACTCGTCGCGCAAGGCGGCGTTCCAGACAATTTCCTCGACGCCGCCGATCGCGCGCAGATGGCTCGCGGAAACCGCGAT
CCACTCGACGGTATTCCCGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A089ZQZ8