Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1753379..1753956 Replicon chromosome
Accession NZ_CP009531
Organism Lactobacillus sp. wkB8

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LACWKB8_RS08170 Protein ID WP_038524459.1
Coordinates 1753624..1753956 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0F4LAE5
Locus tag LACWKB8_RS08165 Protein ID WP_052059690.1
Coordinates 1753379..1753630 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LACWKB8_RS08145 1748496..1749182 + 687 WP_122052583.1 IS3 family transposase -
LACWKB8_RS08150 1749333..1750586 + 1254 WP_038524450.1 Fic family protein -
LACWKB8_RS08155 1750904..1751833 - 930 WP_038524453.1 hypothetical protein -
LACWKB8_RS08160 1751983..1753221 + 1239 WP_038524456.1 MFS transporter -
LACWKB8_RS08165 1753379..1753630 + 252 WP_052059690.1 hypothetical protein Antitoxin
LACWKB8_RS08170 1753624..1753956 + 333 WP_038524459.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LACWKB8_RS08175 1754366..1755622 - 1257 WP_038524462.1 MFS transporter -
LACWKB8_RS08180 1755727..1756575 + 849 WP_038524465.1 hypothetical protein -
LACWKB8_RS09060 1756639..1757250 - 612 WP_052059691.1 substrate-binding domain-containing protein -
LACWKB8_RS08185 1757293..1757541 - 249 WP_052059692.1 LysR family transcriptional regulator -
LACWKB8_RS08190 1757647..1758888 + 1242 WP_038524468.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-107)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12710.84 Da        Isoelectric Point: 9.0990

>T49208 WP_038524459.1 NZ_CP009531:1753624-1753956 [Lactobacillus sp. wkB8]
MVIKQGSILLMDLSPKQGHEQSGKRPYLYLSYKGVEKYAHIAVFAPISTTKRLYPLYQELPAASQTKGVVMLDQLVTIDY
HNQSFQYLEQLPDDYMEKILKIVKVVFQKD

Download         Length: 333 bp

>T49208 NZ_CP009531:1753624-1753956 [Lactobacillus sp. wkB8]
ATGGTAATTAAGCAAGGATCTATTCTATTAATGGATTTAAGTCCTAAACAGGGGCATGAACAATCTGGCAAACGACCTTA
TTTATATTTAAGCTATAAAGGGGTTGAAAAATACGCTCATATTGCTGTGTTTGCTCCTATATCAACTACTAAAAGACTCT
ACCCCTTATATCAAGAATTACCTGCTGCATCTCAAACAAAAGGCGTAGTCATGCTAGATCAGCTAGTTACAATCGATTAT
CATAATCAATCATTTCAATATTTAGAGCAACTACCTGATGACTATATGGAAAAGATACTCAAAATTGTAAAAGTAGTTTT
TCAGAAAGATTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9577.76 Da        Isoelectric Point: 4.4992

>AT49208 WP_052059690.1 NZ_CP009531:1753379-1753630 [Lactobacillus sp. wkB8]
MIRATISKWGNSKGIRLNQAVLQALNATIGDQLNFKIEDNKVIMSKQDDTPDLTFEELFKDYSGEEFDTEIKLPDERKGN
EKW

Download         Length: 252 bp

>AT49208 NZ_CP009531:1753379-1753630 [Lactobacillus sp. wkB8]
ATGATTCGTGCTACTATATCTAAATGGGGTAATTCTAAAGGCATCCGATTAAACCAAGCAGTCTTGCAAGCATTAAACGC
TACTATAGGAGATCAGTTAAACTTTAAAATTGAGGACAACAAAGTAATAATGTCAAAGCAAGATGATACACCTGATTTAA
CTTTTGAAGAACTTTTTAAAGATTATTCAGGTGAAGAATTTGATACTGAAATTAAGTTACCAGATGAGAGAAAAGGAAAT
GAAAAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A0F4LAE5

References