Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-UPF0150
Location 1575364..1575807 Replicon chromosome
Accession NZ_CP009513
Organism Methanosarcina mazei LYC

Toxin (Protein)


Gene name hicA Uniprot ID M1QEU9
Locus tag MSMAL_RS06845 Protein ID WP_011031978.1
Coordinates 1575364..1575588 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0E3RBB6
Locus tag MSMAL_RS06850 Protein ID WP_048131895.1
Coordinates 1575589..1575807 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSMAL_RS06815 (MSMAL_1281) 1570393..1571145 + 753 WP_048040222.1 hypothetical protein -
MSMAL_RS06820 (MSMAL_1282) 1572379..1572726 + 348 WP_011031982.1 hypothetical protein -
MSMAL_RS06825 (MSMAL_1283) 1573074..1573619 + 546 WP_011031981.1 DUF6398 domain-containing protein -
MSMAL_RS19475 (MSMAL_1284) 1574024..1574173 + 150 WP_226987654.1 hypothetical protein -
MSMAL_RS06835 (MSMAL_1285) 1574480..1574839 - 360 WP_011031980.1 DUF6516 family protein -
MSMAL_RS06840 (MSMAL_1286) 1574836..1575156 - 321 WP_226987653.1 hypothetical protein -
MSMAL_RS06845 (MSMAL_1287) 1575364..1575588 - 225 WP_011031978.1 type II toxin-antitoxin system HicA family toxin Toxin
MSMAL_RS06850 (MSMAL_1288) 1575589..1575807 - 219 WP_048131895.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSMAL_RS06855 1576360..1576545 - 186 WP_015410807.1 hypothetical protein -
MSMAL_RS06860 (MSMAL_1289) 1577164..1578249 + 1086 WP_015410806.1 HEAT repeat domain-containing protein -
MSMAL_RS06865 (MSMAL_1290) 1578569..1579864 + 1296 WP_011031974.1 ATP-binding protein -
MSMAL_RS06875 1580409..1580606 - 198 WP_015410804.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1567716..1578249 10533


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-64)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-56)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8175.81 Da        Isoelectric Point: 10.9086

>T49201 WP_011031978.1 NZ_CP009513:c1575588-1575364 [Methanosarcina mazei LYC]
MSGLPVISGMQAIKAFYKAGWLPHRQVGSHVVLRKEGSKVTLTVPKHKELKPGLLRNLIKASGLTIEEFEVLLK

Download         Length: 225 bp

>T49201 NZ_CP009513:c1575588-1575364 [Methanosarcina mazei LYC]
ATGTCTGGACTTCCTGTAATTTCCGGAATGCAGGCAATCAAAGCTTTTTATAAAGCTGGCTGGCTCCCTCACCGGCAGGT
AGGAAGTCATGTTGTCCTTAGAAAGGAAGGATCAAAAGTTACGCTGACTGTCCCGAAGCACAAAGAACTGAAGCCGGGCC
TTTTAAGAAATTTGATAAAAGCTTCAGGGCTGACGATAGAAGAATTCGAAGTTCTTTTAAAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7749.76 Da        Isoelectric Point: 3.7437

>AT49201 WP_048131895.1 NZ_CP009513:c1575807-1575589 [Methanosarcina mazei LYC]
MRFKIILEEDEEVGGFIASCPGLPGCFSQGDTVEEAIENIKEAIQACLESLAEDDLQECIGKSSCRVVDVVA

Download         Length: 219 bp

>AT49201 NZ_CP009513:c1575807-1575589 [Methanosarcina mazei LYC]
ATGCGATTCAAAATTATCCTTGAGGAAGACGAAGAAGTCGGAGGGTTTATTGCCAGCTGTCCCGGTTTACCTGGCTGTTT
TTCACAGGGAGATACTGTAGAAGAAGCTATTGAAAATATTAAAGAAGCGATTCAGGCCTGCCTGGAATCGCTTGCAGAAG
ACGACTTGCAGGAATGCATTGGAAAATCTTCCTGTAGAGTTGTCGATGTGGTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M1QEU9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3RBB6

References