Detailed information of TA system TA049199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-UPF0150
• Location: 1471672..1472115
• Replicon: chromosome
• Accession: NZ_CP009511
• Organism: Methanosarcina mazei SarPi

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: M1QEU9
• Locus tag: MSMAP_RS06425
• Protein ID: WP_011031978.1
• Coordinates: 1471672..1471896 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0E3RBB6
• Locus tag: MSMAP_RS06430
• Protein ID: WP_048131895.1
• Coordinates: 1471897..1472115 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MSMAP_RS06390 (MSMAP_1194)	1467149..1467457	+	309	Protein_1257	helix-turn-helix domain-containing protein	-
MSMAP_RS06395 (MSMAP_1195)	1467783..1468091	+	309	Protein_1258	helix-turn-helix domain-containing protein	-
MSMAP_RS06405 (MSMAP_1197)	1469382..1469927	+	546	WP_011031981.1	DUF6398 domain-containing protein	-
MSMAP_RS18770 (MSMAP_1198)	1470332..1470481	+	150	WP_226987654.1	hypothetical protein	-
MSMAP_RS06415 (MSMAP_1199)	1470788..1471147	-	360	WP_011031980.1	DUF6516 family protein	-
MSMAP_RS06420 (MSMAP_1200)	1471144..1471464	-	321	WP_226987653.1	hypothetical protein	-
MSMAP_RS06425 (MSMAP_1201)	1471672..1471896	-	225	WP_011031978.1	type II toxin-antitoxin system HicA family toxin	Toxin
MSMAP_RS06430 (MSMAP_1202)	1471897..1472115	-	219	WP_048131895.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MSMAP_RS06435 (MSMAP_1203)	1472766..1473071	+	306	WP_048043103.1	nucleotidyltransferase	-
MSMAP_RS06440 (MSMAP_1204)	1473126..1473419	+	294	WP_048043104.1	DUF86 domain-containing protein	-
MSMAP_RS06445 (MSMAP_1205)	1474150..1474737	+	588	WP_048043105.1	class I SAM-dependent methyltransferase	-
MSMAP_RS06450 (MSMAP_1206)	1475350..1475955	+	606	WP_048043106.1	hypothetical protein	-
MSMAP_RS19080	1475977..1476111	+	135	WP_268989007.1	hypothetical protein	-
MSMAP_RS06455 (MSMAP_1207)	1476164..1476382	-	219	WP_048044427.1	type II toxin-antitoxin system HicB family antitoxin	-
MSMAP_RS18485	1476938..1477108	+	171	WP_196296719.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (11-64)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-56)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8175.81 Da
• Isoelectric Point: 10.9086

>T49199 WP_011031978.1 NZ_CP009511:c1471896-1471672 [Methanosarcina mazei SarPi]
MSGLPVISGMQAIKAFYKAGWLPHRQVGSHVVLRKEGSKVTLTVPKHKELKPGLLRNLIKASGLTIEEFEVLLK

Nucleotide

• Length: 225 bp

>T49199 NZ_CP009511:c1471896-1471672 [Methanosarcina mazei SarPi]
ATGTCTGGACTTCCTGTAATTTCCGGAATGCAGGCAATCAAAGCTTTTTATAAAGCTGGCTGGCTCCCTCACCGGCAGGT
AGGAAGTCATGTTGTCCTTAGAAAGGAAGGATCAAAAGTTACGCTGACTGTCCCGAAGCACAAAGAACTGAAGCCGGGCC
TTTTAAGAAATTTGATAAAAGCTTCAGGGCTGACGATAGAAGAATTCGAAGTTCTTTTAAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7749.76 Da
• Isoelectric Point: 3.7437

>AT49199 WP_048131895.1 NZ_CP009511:c1472115-1471897 [Methanosarcina mazei SarPi]
MRFKIILEEDEEVGGFIASCPGLPGCFSQGDTVEEAIENIKEAIQACLESLAEDDLQECIGKSSCRVVDVVA

Nucleotide

• Length: 219 bp

>AT49199 NZ_CP009511:c1472115-1471897 [Methanosarcina mazei SarPi]
ATGCGATTCAAAATTATCCTTGAGGAAGACGAAGAAGTCGGAGGGTTTATTGCCAGCTGTCCCGGTTTACCTGGCTGTTT
TTCACAGGGAGATACTGTAGAAGAAGCTATTGAAAATATTAAAGAAGCGATTCAGGCCTGCCTGGAATCGCTTGCAGAAG
ACGACTTGCAGGAATGCATTGGAAAATCTTCCTGTAGAGTTGTCGATGTGGTTGCTTAA

Structures

Toxin

• AlphaFold DB: M1QEU9

Antitoxin

• AlphaFold DB: A0A0E3RBB6