Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1107189..1107650 Replicon chromosome
Accession NZ_CP009508
Organism Methanosarcina siciliae C2J

Toxin (Protein)


Gene name hicA Uniprot ID A0A0E3PKE7
Locus tag MSSAC_RS04800 Protein ID WP_048170433.1
Coordinates 1107189..1107431 (-) Length 81 a.a.

Antitoxin (Protein)


Gene name hicA Uniprot ID A0A0E3PL86
Locus tag MSSAC_RS04805 Protein ID WP_048170435.1
Coordinates 1107432..1107650 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSSAC_RS04780 1103828..1104181 - 354 WP_156157265.1 hypothetical protein -
MSSAC_RS04785 (MSSAC_0907) 1104395..1104706 - 312 WP_156157266.1 hypothetical protein -
MSSAC_RS04795 (MSSAC_0908) 1105991..1106617 + 627 WP_048180338.1 hypothetical protein -
MSSAC_RS04800 (MSSAC_0909) 1107189..1107431 - 243 WP_048170433.1 type II toxin-antitoxin system HicA family toxin Toxin
MSSAC_RS04805 (MSSAC_0910) 1107432..1107650 - 219 WP_048170435.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSSAC_RS04810 (MSSAC_0911) 1108160..1109248 + 1089 Protein_958 IS110 family transposase -
MSSAC_RS04815 (MSSAC_0912) 1109776..1111254 + 1479 WP_048185671.1 group II intron reverse transcriptase/maturase -
MSSAC_RS25190 (MSSAC_0913) 1111392..1111544 + 153 Protein_960 IS110 family transposase -
MSSAC_RS23895 1111806..1112200 + 395 Protein_961 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1104395..1135736 31341


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(10-65)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-57)


Sequences

Toxin        


Download         Length: 81 a.a.        Molecular weight: 9169.99 Da        Isoelectric Point: 11.0632

>T49198 WP_048170433.1 NZ_CP009508:c1107431-1107189 [Methanosarcina siciliae C2J]
MSKILPLPAKKVVKALENIGFEQIRQKGSHLFLRHPDGRTTIVPMHPTEKIGSGMINKIIKDAKITRNEWIELVNRLILF

Download         Length: 243 bp

>T49198 NZ_CP009508:c1107431-1107189 [Methanosarcina siciliae C2J]
ATGAGTAAAATATTACCTCTTCCTGCAAAAAAAGTTGTAAAAGCACTTGAAAATATAGGTTTCGAGCAGATAAGGCAGAA
GGGTAGTCACTTATTTTTACGGCATCCAGATGGAAGGACCACTATAGTCCCAATGCACCCAACAGAAAAAATCGGAAGCG
GAATGATCAATAAAATAATAAAAGATGCAAAAATTACACGGAATGAATGGATTGAATTGGTAAATAGACTTATTCTTTTC
TAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8315.32 Da        Isoelectric Point: 3.9575

>AT49198 WP_048170435.1 NZ_CP009508:c1107650-1107432 [Methanosarcina siciliae C2J]
MKEFTVLIEQDENGIYVASVPELPGCHTQAETLDELNRRIKEAIELYLEVEAEEEEENHLDFIGIQKVKVEV

Download         Length: 219 bp

>AT49198 NZ_CP009508:c1107650-1107432 [Methanosarcina siciliae C2J]
ATGAAAGAGTTTACCGTCTTAATCGAACAAGACGAAAATGGAATTTACGTGGCTTCAGTTCCAGAACTTCCAGGATGCCA
TACACAAGCTGAAACGCTTGACGAATTAAACAGAAGAATAAAAGAGGCAATAGAACTTTACCTTGAAGTTGAAGCCGAGG
AAGAGGAAGAAAATCATCTTGATTTCATCGGAATACAGAAAGTTAAAGTTGAGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E3PKE7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3PL86

References