Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1033586..1034047 Replicon chromosome
Accession NZ_CP009507
Organism Methanosarcina siciliae HI350

Toxin (Protein)


Gene name hicA Uniprot ID A0A0E3PKE7
Locus tag MSSIH_RS04470 Protein ID WP_048170433.1
Coordinates 1033586..1033828 (-) Length 81 a.a.

Antitoxin (Protein)


Gene name hicA Uniprot ID A0A0E3PL86
Locus tag MSSIH_RS04475 Protein ID WP_048170435.1
Coordinates 1033829..1034047 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSSIH_RS04450 1030209..1030562 - 354 WP_148704935.1 hypothetical protein -
MSSIH_RS04455 (MSSIH_0848) 1030775..1031086 - 312 WP_148704936.1 hypothetical protein -
MSSIH_RS04460 1032131..1032373 + 243 WP_048170428.1 hypothetical protein -
MSSIH_RS04465 (MSSIH_0849) 1032387..1033013 + 627 WP_148704937.1 hypothetical protein -
MSSIH_RS04470 (MSSIH_0850) 1033586..1033828 - 243 WP_048170433.1 type II toxin-antitoxin system HicA family toxin Toxin
MSSIH_RS04475 (MSSIH_0851) 1033829..1034047 - 219 WP_048170435.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSSIH_RS04480 1034437..1034829 + 393 Protein_886 transposase -
MSSIH_RS04485 1034847..1035944 - 1098 Protein_887 IS630 family transposase -
MSSIH_RS04490 1036001..1036357 + 357 Protein_888 transposase -
MSSIH_RS04495 (MSSIH_0855) 1037717..1038613 + 897 WP_148704938.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(10-65)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-57)


Sequences

Toxin        


Download         Length: 81 a.a.        Molecular weight: 9169.99 Da        Isoelectric Point: 11.0632

>T49197 WP_048170433.1 NZ_CP009507:c1033828-1033586 [Methanosarcina siciliae HI350]
MSKILPLPAKKVVKALENIGFEQIRQKGSHLFLRHPDGRTTIVPMHPTEKIGSGMINKIIKDAKITRNEWIELVNRLILF

Download         Length: 243 bp

>T49197 NZ_CP009507:c1033828-1033586 [Methanosarcina siciliae HI350]
ATGAGTAAAATATTACCTCTTCCTGCAAAAAAAGTTGTAAAAGCACTTGAAAATATAGGTTTCGAGCAGATAAGGCAGAA
GGGTAGTCACTTATTTTTACGGCATCCAGATGGAAGGACCACTATAGTCCCAATGCACCCGACAGAAAAAATCGGAAGCG
GAATGATCAATAAAATAATAAAAGATGCAAAAATTACACGGAATGAATGGATTGAATTGGTAAATAGACTTATTCTTTTC
TAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8315.32 Da        Isoelectric Point: 3.9575

>AT49197 WP_048170435.1 NZ_CP009507:c1034047-1033829 [Methanosarcina siciliae HI350]
MKEFTVLIEQDENGIYVASVPELPGCHTQAETLDELNRRIKEAIELYLEVEAEEEEENHLDFIGIQKVKVEV

Download         Length: 219 bp

>AT49197 NZ_CP009507:c1034047-1033829 [Methanosarcina siciliae HI350]
ATGAAAGAGTTTACCGTCTTAATCGAACAGGACGAAAATGGAATTTACGTGGCTTCAGTTCCAGAACTTCCAGGATGCCA
TACACAAGCTGAAACGCTTGACGAATTAAACAGAAGAATAAAAGAGGCAATAGAACTTTACCTTGAAGTTGAAGCCGAGG
AAGAGGAAGAAAATCATCTTGATTTCATCGGAATACAGAAAGTTAAAGTTGAGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E3PKE7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3PL86

References