Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1062481..1062942 Replicon chromosome
Accession NZ_CP009506
Organism Methanosarcina siciliae T4/M

Toxin (Protein)


Gene name hicA Uniprot ID A0A0E3PKE7
Locus tag MSSIT_RS04660 Protein ID WP_048170433.1
Coordinates 1062481..1062723 (-) Length 81 a.a.

Antitoxin (Protein)


Gene name hicA Uniprot ID A0A0E3PL86
Locus tag MSSIT_RS04665 Protein ID WP_048170435.1
Coordinates 1062724..1062942 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSSIT_RS21650 1057584..1058681 + 1098 Protein_914 IS630 family transposase -
MSSIT_RS04640 1059064..1059411 - 348 WP_048170424.1 hypothetical protein -
MSSIT_RS04645 (MSSIT_0880) 1059630..1059941 - 312 WP_148704936.1 hypothetical protein -
MSSIT_RS04650 1061026..1061268 + 243 WP_048170428.1 hypothetical protein -
MSSIT_RS04655 (MSSIT_0883) 1061282..1061908 + 627 WP_048170430.1 hypothetical protein -
MSSIT_RS04660 (MSSIT_0884) 1062481..1062723 - 243 WP_048170433.1 type II toxin-antitoxin system HicA family toxin Toxin
MSSIT_RS04665 (MSSIT_0885) 1062724..1062942 - 219 WP_048170435.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSSIT_RS21655 1063332..1063724 + 393 Protein_921 transposase -
MSSIT_RS21660 1063742..1064839 - 1098 Protein_922 IS630-like element ISMac17 family transposase -
MSSIT_RS21665 1064896..1065252 + 357 Protein_923 transposase -
MSSIT_RS04685 (MSSIT_0889) 1066612..1067508 + 897 WP_148704938.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(10-65)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-57)


Sequences

Toxin        


Download         Length: 81 a.a.        Molecular weight: 9169.99 Da        Isoelectric Point: 11.0632

>T49196 WP_048170433.1 NZ_CP009506:c1062723-1062481 [Methanosarcina siciliae T4/M]
MSKILPLPAKKVVKALENIGFEQIRQKGSHLFLRHPDGRTTIVPMHPTEKIGSGMINKIIKDAKITRNEWIELVNRLILF

Download         Length: 243 bp

>T49196 NZ_CP009506:c1062723-1062481 [Methanosarcina siciliae T4/M]
ATGAGTAAAATATTACCTCTTCCTGCAAAAAAAGTTGTAAAAGCACTTGAAAATATAGGTTTCGAGCAGATAAGGCAGAA
GGGTAGTCACTTATTTTTACGGCATCCAGATGGAAGGACCACTATAGTCCCAATGCACCCGACAGAAAAAATCGGAAGCG
GAATGATCAATAAAATAATAAAAGATGCAAAAATTACACGGAATGAATGGATTGAATTGGTAAATAGACTTATTCTTTTC
TAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8315.32 Da        Isoelectric Point: 3.9575

>AT49196 WP_048170435.1 NZ_CP009506:c1062942-1062724 [Methanosarcina siciliae T4/M]
MKEFTVLIEQDENGIYVASVPELPGCHTQAETLDELNRRIKEAIELYLEVEAEEEEENHLDFIGIQKVKVEV

Download         Length: 219 bp

>AT49196 NZ_CP009506:c1062942-1062724 [Methanosarcina siciliae T4/M]
ATGAAAGAGTTTACCGTCTTAATCGAACAGGACGAAAATGGAATTTACGTGGCTTCAGTTCCAGAACTTCCAGGATGCCA
TACACAAGCTGAAACGCTTGACGAATTAAACAGAAGAATAAAAGAGGCAATAGAACTTTACCTTGAAGTTGAAGCCGAGG
AAGAGGAAGAAAATCATCTTGATTTCATCGGAATACAGAAAGTTAAAGTTGAGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E3PKE7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3PL86

References