Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-UPF0150
Location 2127851..2128294 Replicon chromosome
Accession NZ_CP009504
Organism Methanosarcina sp. WH1

Toxin (Protein)


Gene name hicA Uniprot ID A0A0E3KXN0
Locus tag MSWH1_RS09310 Protein ID WP_011307322.1
Coordinates 2128070..2128294 (+) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0E3NRH1
Locus tag MSWH1_RS09305 Protein ID WP_048127971.1
Coordinates 2127851..2128069 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSWH1_RS09275 2123544..2123729 + 186 WP_048127965.1 hypothetical protein -
MSWH1_RS20100 2124478..2125129 + 652 Protein_1828 helix-turn-helix domain-containing protein -
MSWH1_RS09295 (MSWH1_1729) 2125330..2126043 - 714 WP_231585673.1 methyltransferase domain-containing protein -
MSWH1_RS09300 (MSWH1_1730) 2126326..2127153 + 828 WP_048127969.1 TrmB family transcriptional regulator -
MSWH1_RS18175 2127381..2127509 - 129 Protein_1831 DNA polymerase III subunit beta -
MSWH1_RS09305 (MSWH1_1731) 2127851..2128069 + 219 WP_048127971.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSWH1_RS09310 (MSWH1_1732) 2128070..2128294 + 225 WP_011307322.1 type II toxin-antitoxin system HicA family toxin Toxin
MSWH1_RS09315 2128561..2128815 - 255 WP_048127973.1 hypothetical protein -
MSWH1_RS09320 (MSWH1_1733) 2129147..2130406 + 1260 WP_052723160.1 hypothetical protein -
MSWH1_RS09325 2130825..2131019 + 195 WP_048127975.1 type II toxin-antitoxin system HicA family toxin -
MSWH1_RS09330 (MSWH1_1734) 2131022..2131264 + 243 WP_048127977.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-64)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-55)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8085.68 Da        Isoelectric Point: 11.0775

>T49194 WP_011307322.1 NZ_CP009504:2128070-2128294 [Methanosarcina sp. WH1]
MSGLPVISGMQAIKAFSKAGWLPHRQVGSHVVLRKEGSKVTLTVPKHKELKPGLLRNLIKVSGLTVEEFEALLK

Download         Length: 225 bp

>T49194 NZ_CP009504:2128070-2128294 [Methanosarcina sp. WH1]
ATGTCTGGGCTTCCTGTAATTTCCGGAATGCAGGCAATTAAAGCCTTTTCTAAAGCTGGCTGGCTCCCTCACCGACAGGT
GGGCAGTCATGTTGTCCTTAGAAAAGAAGGCTCAAAAGTTACGCTGACGGTCCCGAAGCACAAAGAACTGAAGCCGGGTC
TTTTAAGAAACTTGATAAAAGTTTCAGGACTTACTGTAGAAGAATTCGAAGCTCTTTTGAAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7819.89 Da        Isoelectric Point: 3.7558

>AT49194 WP_048127971.1 NZ_CP009504:2127851-2128069 [Methanosarcina sp. WH1]
MRFKIILEEDEEVVGFIASCPGLPGCFSQGDTVEEAIENIKEAIQACLESLAEDELQECIGKSSCRVIDVVA

Download         Length: 219 bp

>AT49194 NZ_CP009504:2127851-2128069 [Methanosarcina sp. WH1]
ATGCGGTTCAAAATTATCCTTGAGGAAGATGAAGAAGTCGTGGGATTTATTGCCAGCTGCCCAGGCTTACCGGGATGTTT
TTCACAGGGAGATACTGTAGAAGAAGCTATTGAAAACATTAAAGAAGCAATTCAAGCCTGTCTGGAATCTCTTGCAGAAG
ACGAACTGCAGGAATGTATTGGGAAATCTTCCTGTAGAGTTATTGACGTGGTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E3KXN0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3NRH1

References