Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-UPF0150
Location 1932255..1932698 Replicon chromosome
Accession NZ_CP009503
Organism Methanosarcina sp. WWM596

Toxin (Protein)


Gene name hicA Uniprot ID A0A0E3NKU0
Locus tag MSWHS_RS08520 Protein ID WP_048158931.1
Coordinates 1932255..1932479 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0E3NJU3
Locus tag MSWHS_RS08525 Protein ID WP_048158932.1
Coordinates 1932480..1932698 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MSWHS_RS20195 1928167..1928334 + 168 WP_156151203.1 hypothetical protein -
MSWHS_RS08500 (MSWHS_1579) 1929267..1929509 - 243 WP_048127977.1 type II toxin-antitoxin system HicB family antitoxin -
MSWHS_RS08505 1929512..1929706 - 195 WP_048127975.1 type II toxin-antitoxin system HicA family toxin -
MSWHS_RS08510 (MSWHS_1580) 1930125..1931402 - 1278 WP_052722661.1 hypothetical protein -
MSWHS_RS08515 1931734..1931988 + 255 WP_048127973.1 hypothetical protein -
MSWHS_RS08520 (MSWHS_1581) 1932255..1932479 - 225 WP_048158931.1 type II toxin-antitoxin system HicA family toxin Toxin
MSWHS_RS08525 (MSWHS_1582) 1932480..1932698 - 219 WP_048158932.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
MSWHS_RS19225 1933040..1933168 + 129 Protein_1704 DNA polymerase III subunit beta -
MSWHS_RS08530 (MSWHS_1583) 1933611..1934855 + 1245 WP_048158641.1 IS110 family transposase -
MSWHS_RS08535 (MSWHS_1584) 1935020..1935847 - 828 WP_048158933.1 TrmB family transcriptional regulator -
MSWHS_RS08540 (MSWHS_1585) 1936130..1936843 + 714 WP_231585673.1 methyltransferase domain-containing protein -
MSWHS_RS21460 1937044..1937695 - 652 Protein_1708 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(11-64)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-55)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8057.63 Da        Isoelectric Point: 10.8877

>T49193 WP_048158931.1 NZ_CP009503:c1932479-1932255 [Methanosarcina sp. WWM596]
MSGLPVISGMQAIKAFSKAGWLPHQQVGSHVVLRKEGSKVTLTVPKHKELKPGLLRNLIKVSGLTVEEFEALLK

Download         Length: 225 bp

>T49193 NZ_CP009503:c1932479-1932255 [Methanosarcina sp. WWM596]
ATGTCTGGGCTTCCTGTAATTTCCGGAATGCAGGCAATTAAAGCCTTTTCTAAAGCTGGCTGGCTCCCTCACCAACAGGT
GGGCAGTCATGTTGTCCTTAGAAAAGAAGGCTCAAAAGTTACGCTGACGGTCCCGAAGCACAAAGAACTGAAGCCGGGTC
TTTTAAGAAACTTGATAAAAGTTTCAGGACTTACTGTAGAAGAATTCGAAGCTCTTTTGAAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 7777.81 Da        Isoelectric Point: 3.7558

>AT49193 WP_048158932.1 NZ_CP009503:c1932698-1932480 [Methanosarcina sp. WWM596]
MRFKIILEEDEEVGGFIASCPGLPGCFSQGDTVEEAIENIKEAIQACLESLAEDELQECIGKSSCRVIDVVA

Download         Length: 219 bp

>AT49193 NZ_CP009503:c1932698-1932480 [Methanosarcina sp. WWM596]
ATGCGGTTCAAAATTATCCTTGAGGAAGATGAAGAAGTCGGGGGATTTATTGCCAGCTGCCCAGGCTTACCGGGATGTTT
TTCACAGGGAGATACTGTAGAAGAAGCTATTGAAAACATTAAAGAAGCAATTCAAGCCTGTCTGGAATCTCTTGCAGAAG
ACGAACTGCAGGAATGTATTGGGAAATCTTCCTGTAGAGTTATTGACGTGGTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E3NKU0

Antitoxin

Source ID Structure
AlphaFold DB A0A0E3NJU3

References