Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 4528933..4529474 Replicon chromosome
Accession NZ_CP009494
Organism Mycolicibacterium smegmatis MC2 155

Toxin (Protein)


Gene name mazF Uniprot ID A0A2U9PVI3
Locus tag LJ00_RS21755 Protein ID WP_003895797.1
Coordinates 4529145..4529474 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0R0N3
Locus tag LJ00_RS21750 Protein ID WP_011729843.1
Coordinates 4528933..4529142 (+) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LJ00_RS21725 4524204..4524974 - 771 WP_011729837.1 SDR family oxidoreductase -
LJ00_RS21730 4525004..4525912 - 909 WP_011729838.1 cupin domain-containing protein -
LJ00_RS21735 4525942..4526862 - 921 WP_011729840.1 NADP-dependent oxidoreductase -
LJ00_RS21740 4526952..4527143 - 192 Protein_4256 hypothetical protein -
LJ00_RS21745 4527360..4528772 + 1413 Protein_4257 dihydrolipoyl dehydrogenase -
LJ00_RS21750 4528933..4529142 + 210 WP_011729843.1 antitoxin MazE family protein Antitoxin
LJ00_RS21755 4529145..4529474 + 330 WP_003895797.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LJ00_RS21760 4529517..4530080 - 564 WP_014878038.1 TetR/AcrR family transcriptional regulator -
LJ00_RS21765 4530186..4531121 + 936 WP_003895799.1 alpha/beta fold hydrolase -
LJ00_RS21770 4531118..4532830 + 1713 WP_011729844.1 NAD(P)/FAD-dependent oxidoreductase -
LJ00_RS21780 4533047..4533427 + 381 WP_029104585.1 phage holin family protein -
LJ00_RS21785 4533445..4534470 + 1026 WP_014878040.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(5-68)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 11739.52 Da        Isoelectric Point: 10.4023

>T49180 WP_003895797.1 NZ_CP009494:4529145-4529474 [Mycolicibacterium smegmatis MC2 155]
VRRGDIYTAAARGAYTGKPRPVLIIQDDRFDATASVTVVPFTTSDTDAPLLRIRIEPTPATGLSTVSSLMIDKVTTVPRS
SLTHRVGRLAETDMVRTDRALLVFLGIAG

Download         Length: 330 bp

>T49180 NZ_CP009494:4529145-4529474 [Mycolicibacterium smegmatis MC2 155]
GTGCGGCGCGGCGATATCTACACCGCGGCGGCGCGAGGTGCGTACACCGGCAAGCCTCGGCCGGTCCTCATCATCCAGGA
CGACCGATTCGATGCCACCGCGTCGGTCACCGTCGTGCCGTTTACGACAAGCGACACCGACGCACCGCTTCTGCGAATTC
GAATCGAGCCAACGCCAGCCACCGGACTCTCCACGGTCAGCTCGTTGATGATCGACAAGGTGACCACAGTTCCCCGCTCC
AGCTTGACGCACCGTGTCGGCCGACTGGCGGAGACAGACATGGTCAGGACCGACCGCGCACTCCTGGTTTTTCTGGGAAT
CGCCGGGTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 8114.98 Da        Isoelectric Point: 6.2231

>AT49180 WP_011729843.1 NZ_CP009494:4528933-4529142 [Mycolicibacterium smegmatis MC2 155]
MTPARDRVRRHRERLRRQGLRPVQIWVPDVNAPEFRREAHRQSELVAAGEHEAEDQAFVDAISVDWDDA

Download         Length: 210 bp

>AT49180 NZ_CP009494:4528933-4529142 [Mycolicibacterium smegmatis MC2 155]
ATGACTCCCGCGCGTGACCGAGTACGCCGACATCGCGAGCGGCTGCGGCGACAAGGTCTGCGGCCGGTGCAGATCTGGGT
CCCCGACGTGAATGCACCCGAATTTCGCCGGGAGGCGCACCGGCAGTCTGAGTTGGTCGCCGCCGGCGAGCATGAGGCCG
AAGATCAAGCTTTCGTCGACGCGATCTCCGTCGACTGGGACGACGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U9PVI3

Antitoxin

Source ID Structure
AlphaFold DB A0R0N3

References